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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1,302 - 1,312 (11page)
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Purpose TRIM29 overexpression has been reported in several human malignancies and showed correlation with cancer cell malignancy. The aim of the current study is to examine its clinical significance and biological roles in human bladder cancer tissues and cell lines.
Materials and Methods A total of 102 cases of bladder cancer tissues were examined for TRIM29 expression by immunohistochemistry. siRNA and plasmid transfection were performed in 5637 and BIU- 87 cell lines. Cell Counting Kit-8, flow cytometry, western blot, and real-time polymerase chain reaction were performed to examine its biological roles and mechanism in bladder cancer cells.
Results We found that TRIM29 overexpression showed correlation with invading depth (p=0.0087).
Knockdown of TRIM29 expression in bladder cancer cell line 5637 inhibited cell growth rate and cell cycle transition while its overexpression in BIU-87 cells accelerated cell proliferation and cell cycle progression. TRIM29 overexpression also inhibited cell apoptosis induced by cisplatin. In addition, we demonstrated that TRIM29 depletion decreased while its overexpression led to upregulated expression of cyclin D1, cyclin E, and Bcl-2. We also showed that TRIM29 knockdown inhibited protein kinase C (PKC) and nuclear factor B (NF-B) signaling while its overexpression stimulated the PKC and NF-B pathways. BAY 11-7082 (NF-B inhibitor) partly attenuated the effect of TRIM29 on expression of cyclin and Bcl-2. Treatment with PKC inhibitor staurosporine resulted in ameliorated TRIM29 induced activation of NF-B.
Conclusion The current study demonstrated that TRIM29 upregulates cyclin and Bcl family proteins level to facilitate malignant cell growth and inhibit drug-induced apoptosis in bladder cancer, possibly through PKC–NF-B signaling pathways.
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참고문헌 (19)
참고문헌 신청
Siegel R / 2012 / Cancer statistics, 2012 / CA Cancer J Clin 62:10~29
Kosaka Y / 2007 / Tripartite motif-containing 29(TRIM29)is a novel marker for lymph node metastasis in gastric cancer / Ann Surg Oncol 14:2543~2549
Li Q / 2011 / Tripartite motif 8(TRIM8)modulates TNFalpha-and IL-1beta-triggered NF-kappaB activation by targeting TAK1 for K63-linked polyubiquitination / Proc Natl Acad Sci U S A 108:19341~19346
Noguchi K / 2011 / TRIM40 promotes neddylation of IKKgamma and is downregulated in gastrointestinal cancers / Carcinogenesis 32:995~1004
Tang ZP / 2013 / Ataxia-telangiectasia group D complementing gene (ATDC) promotes lung cancer cell proliferation by activating NF-kappaB pathway / PLoS One 8:63676
Kosaka Y / 2007 / Tripartite motif-containing 29(TRIM29)is a novel marker for lymph node metastasis in gastric cancer / Ann Surg Oncol 14:2543~2549
Li Q / 2011 / Tripartite motif 8(TRIM8)modulates TNFalpha-and IL-1beta-triggered NF-kappaB activation by targeting TAK1 for K63-linked polyubiquitination / Proc Natl Acad Sci U S A 108:19341~19346
Noguchi K / 2011 / TRIM40 promotes neddylation of IKKgamma and is downregulated in gastrointestinal cancers / Carcinogenesis 32:995~1004
Tang ZP / 2013 / Ataxia-telangiectasia group D complementing gene (ATDC) promotes lung cancer cell proliferation by activating NF-kappaB pathway / PLoS One 8:63676
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