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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 175 - 180 (6page)
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초록· 키워드
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Purpose: Calcium ionophore (CI) is used to generate dendritic cells (DCs) from progenitor cells, monocytes, or leukemic cells. The aim of this study was to determine the optimal dose of CI and the appropriate length of cell culture required for acute myeloid leukemia (AML) cells and to evaluate the limitations associated with CI.
Materials and Methods: To generate leukemic DCs, leukemic cells (4×106 cells) from six AML patients were cultured with various concentrations of CI and/or IL-4 for 1, 2 or 3 days.
Results: Potent leukemic DCs were successfully generated from all AML patients, with an average number of 1.2×106 cells produced in the presence of CI (270 ng/ml) for 2 days. Several surface molecules were clearly upregulated in AML cells supplemented with CI and IL-4, but not CD11c. Leukemic DCs cultured with CI had a higher allogeneic T cell stimulatory capacity than untreated AML cells, but the addition of IL-4 did not augment the MLR activity of these cells. AML cells cultured with CI in the presence or absence of IL-4 showed increased levels of apoptosis in comparison to primary cultures of AML cells.
Conclusion: Although CI appears to be advantageous in terms of time and cost effectiveness, the results of the present study suggest that the marked induction of apoptosis by CI limits its application to the generation of DCs from AML cells. (Cancer Res Treat. 2007;39:175-180)
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참고문헌 (23)
참고문헌 신청
Banchereau J / 1998 / Dendritic cells and the control of immunity 392 : 245 ~ 252
Buggins AG / 1999 / Effect of costimulation and the microenvironment on antigen presentation by leukemic cells 94 : 3479 ~ 3490
Choi BH / 2006 / Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemia cells for clinical immunotherap
Coppock DL / / Kern J. Identification of a malignant counterpart of the monocyte- dendritic cell progenitor in an acute myeloid leukemia. Blood. 1994;84 : 3054 ~ 62
Czerniecki BJ / 1997 / Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic cells 159 : 3823 ~ 383
Buggins AG / 1999 / Effect of costimulation and the microenvironment on antigen presentation by leukemic cells 94 : 3479 ~ 3490
Choi BH / 2006 / Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemia cells for clinical immunotherap
Coppock DL / / Kern J. Identification of a malignant counterpart of the monocyte- dendritic cell progenitor in an acute myeloid leukemia. Blood. 1994;84 : 3054 ~ 62
Czerniecki BJ / 1997 / Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic cells 159 : 3823 ~ 383
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