Component-resolved diagnosis using microarray for diagnosing hypersensitivity to raw fruits in birch pollen sensitized children

최원복; 유정섭; 이윤영; 정수인; 송준섭; 양승; 황일태; 이하백; 백혜성

Component-resolved diagnosis using microarray for diagnosing hypersensitivity to raw fruits in birch pollen sensitized children

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자작나무에 감작된 소아에서 과일 알레르기를 진단하기 위한 microarray법에 의한 성분 항원검사

Type: Academic journal

Author: 최원복 유정섭 이윤영 정수인 송준섭 양승 황일태 이하백 백혜성

Journal: 대한소아알레르기호흡기학회 Allergy Asthma & Respiratory Diseases Allergy Asthma & Respiratory Diseases 제3권 제3호 KCI Accredited Journals

Published: 2015.1

Pages: 200 - 205 (6page)

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Component-resolved diagnosis using microarray for diagnosing hypersensitivity to raw fruits in birch pollen sensitized children

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Purpose: Recently, component-resolved diagnosis (CRD) using microarray technology has been introduced to the field of clinical allergy. This study was aimed to investigate the clinical usefulness of microarray-based IgE detection for diagnosing clinical raw fruit allergy in birch pollen-sensitized children. Methods: Thirty-one children with allergic disease who had been sensitized to pollen were studied. A pollen-sensitized patient was defined as having an allergen-specific history with concomitant positive skin-prick tests (SPTs) to natural allergen extracts or positive allergen-specific IgE. All subjects underwent SPTs for pollen and fruit. In all subjects, specific IgE to pollen and fruit were measured by ImmunoCAP. Specific IgE antibodies to allergen components were determined by a customized allergen microarray (ISAC). Results: Thirteen of the 31 patients (41.9%) had a history of fruit hypersensitivity with positive SPTs. Measuring IgE to allergen components by ISAC, all the 13 patients with fruit hypersensitivity were positive to at least one of Mal d 1, Pru p 1, Pru p 3, Act d 8, and Act d 2 compared to 12 of the 13 patients (92.3%) who had at least 1 positive IgE to fruits (apple, peach, and kiwi) using ImmunoCAP. The sensitivity of ISAC microarray was 100.0% for the diagnosis of fruit hypersensitivity, but its specificity was 27.7% (5/18). The sensitivity of ImmunoCAP was 92.3%, and its specificity was 83.3%. Conclusion: The sensitivity of allergen components tested using microarray for the diagnosis of clinical fruit hypersensitivity in children with pollen allergy was high; however, its specificity was low.

#Allergen component #Fruit allergy #Birch allergy #Microarray

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Asero R / 2013 / Anaphylaxis to plant-foods and pollen allergens in patients with lipid transfer protein syndrome / Curr Opin Allergy Clin Immunol 13 : 379 ~ 385

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