In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

김규호; Suk Jin Choi; 최연일; Lucia Kim; Insuh Park; Jeeyoung Han; Joon Mee Kim; CHU YOUNG CHAE

In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

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Type: Academic journal

Author: 김규호 Suk Jin Choi 최연일 Lucia Kim Insuh Park Jeeyoung Han Joon Mee Kim CHU YOUNG CHAE

Journal: 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제47권 제3호 KCI Accredited Journals

Published: 2013.1

Pages: 238 - 244 (7page)

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In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

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Background: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. Methods: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. Results: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. Conclusions: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks.

#Tissue array analysis #Sepharose #Paraffin #Pathology #Research

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