지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 119 - 123 (5page)
이용수
초록· 키워드
오류제보하기
Acetylcholine receptors (AChR) including muscarinic and nicotinic AChR are widely expressed and mediate a variety of physiological cellular responses in neuronal and non-neuronal cells. Notably, a functional cholinergic system exists in oral epithelial cells, and nicotinic AChR (nAChR) mediates cholinergic anti-inflammatory responses. However, the pathophysiological roles of AChR in periodontitis are unclear.
Here, we show that activation of AChR elicits increased cytosolic Ca2+ ([Ca2+]i), transient cytotoxicity, and induction of receptor activator of nuclear factor kappa-B ligand (RANKL) expression. Intracellular Ca2+ mobilization in human gingival fibroblast-1 (hGF-1) cells was measured using the fluorescent Ca2+ indicator, fura-2/AM. Cytotoxicity and induction of gene expression were evaluated by measuring the release of glucose-6-phosphate dehydrogenase and RT-PCR. Activation of AChR in hGF-1 cells by carbachol (Cch) induced [Ca2+]i increase in a dose-dependent manner.
Treatment with a high concentration of Cch on hGF-1 cells caused transient cytotoxicity. Notably, treatment of hGF-1 cells with Cch resulted in upregulated RANKL expression.
The findings may indicate potential roles of AChR in gingival fibroblast cells in bone remodeling.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Nachmansohn D / 1959 / Role of acetylcholine in axonal conduction and neuromuscular transmission / Am J Phys Med 38:190~206
Wessler I / 2003 / The non-neuronal cholinergic system in humans: expression, function and pathophysiology / Life Sci 72(18-19):2055~2061
I Wessler / 2008 / Acetylcholine beyond neurons: the non-neuronal cholinergic system in humans / British Journal of Pharmacology 154(8):1558~1571
Nguyen VT / 2000 / Choline acetyltransferase, acetylcholinesterase, and nicotinic acetylcholine receptors of human gingival and esophageal epithelia / J Dent Res 79(4):939~949
Pavlov VA / 2003 / The cholinergic anti-inflammatory pathway: a missing link in neuroimmunomodulation / Mol Med 9(5-8):125~134
Wessler I / 2003 / The non-neuronal cholinergic system in humans: expression, function and pathophysiology / Life Sci 72(18-19):2055~2061
I Wessler / 2008 / Acetylcholine beyond neurons: the non-neuronal cholinergic system in humans / British Journal of Pharmacology 154(8):1558~1571
Nguyen VT / 2000 / Choline acetyltransferase, acetylcholinesterase, and nicotinic acetylcholine receptors of human gingival and esophageal epithelia / J Dent Res 79(4):939~949
Pavlov VA / 2003 / The cholinergic anti-inflammatory pathway: a missing link in neuroimmunomodulation / Mol Med 9(5-8):125~134
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Hypoxia Inducible Factor-1α Directly Induces the Expression of Receptor Activator of Nuclear Factor-κB Ligand in Chondrocytes
International Journal of Oral Biology
2016 .01
-
Isoliquiritigenin Inhibits Metastatic Breast Cancer Cell-induced Receptor Activator of Nuclear Factor Kappa-B Ligand/Osteoprotegerin Ratio in Human Osteoblastic Cells
대한암예방학회지
2015 .01
-
P2Y<sub>6</sub> 수용체 길항제의 파골세포 분화 촉진 효과 규명
약학회지
2015 .01
-
Molecular determinants of α3β4 nicotinic acetylcholine receptors inhibition by triterpenoids
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Melatonin inhibits nicotinic acetylcholine receptor functions in bovine chromaffin cells
International Journal of Oral Biology
2019 .01
-
Updated review of resistance to neuromuscular blocking agents
Anesthesia and Pain Medicine
2018 .01
-
Short-chain fatty acids, including acetate, propionate, and butyrate, elicit differential regulation of intracellular Ca2+ mobilization, expression of IL-6 and IL-8, and cell viability in gingival fibroblast cells
International Journal of Oral Biology
2020 .01
-
Hydrogen peroxide attenuates refilling of intracellular calcium store in mouse pancreatic acinar cells
The Korean Journal of Physiology & Pharmacology
2017 .03
-
House Dust Mite Extract Induces PLC/IP3-dependent Ca2+ Signaling and IL-8 Expression in Human Gingival Epithelial Cells
International Journal of Oral Biology
2015 .01
-
Expression of Senescence-Associated Secretory Phenotype in Senescent Gingival Fibroblasts
치위생과학회지
2023 .06
-
The correlation of muscarinic acetylcholine 1 receptor with status epilepticus in the hippocampus
대한체질인류학회 학술대회 연제 초록
2017 .06
-
Expression of neurotransmitter receptors in oral keratinocytes and their response to agonists
International Journal of Oral Biology
2021 .01
-
Efficacy of Gaze Photographs in Diagnosing Ocular Myasthenia Gravis
Journal of Clinical Neurology
2018 .01
-
MSK1 regulates RANKL-induced NFATc1 expression through CREB and c-Fos
Journal of Biomedical and Translational Research
2015 .01
-
Attenuation of RANKL-induced Osteoclast Formation via p38-mediated NFATc1 Signaling Pathways by Extract of Euphorbia Lathyris L
대한골대사학회지
2016 .01
-
The functional relevance of muscarinic acetylcholine receptor and Phospholipase C (PLC) beta expression following status epilepticus in the hippocampus
대한체질인류학회 학술대회 연제 초록
2015 .05
-
Neuroprotective Effect of Duloxetine on Chronic Cerebral Hypoperfusion-Induced Hippocampal Neuronal Damage
Biomolecules & Therapeutics(구 응용약물학회지)
2018 .01
-
한천중층시험법을 이용한 다양한 세포주의 티타늄 세포독성 비교
대한구강해부학회지
2017 .12
-
Hepatocyte Growth Factor-mediated Regulation of OCT4 in human Mesenchymal Stem Cells
대한의생명과학회지
2024 .09
-
Clonal Expansion of Allergen-specific CD4+ T Cell in the Lung in the Absence of Lymph Nodes
Immune Network
2017 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0