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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 242 - 249 (8page)
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This study was conducted to develop an SNP set that can be useful for marker-assisted breeding (MAB) in watermelon (Citrullus. lanatus L) using Genotyping-bysequencing (GBS) analysis of 20 commercial elite watermelon inbreds. The result of GBS showed that 77% of approximately 1.1 billion raw reads were mapped on the watermelon genome with an average mapping region of about 4,000 Kb, which indicated genome coverage of 2.3%. After the filtering process, a total of 2,670 SNPs with an average depth of 31.57 and the PIC (Polymorphic Information Content) value of 0.1~0.38 for 20 elite inbreds were obtained. Among those SNPs, 55 SNPs (5 SNPs per chromosome that are equally distributed on each chromosome) were selected. For the understanding genetic relationship of 20 elite inbreds, PCA (Principal Component Analysis) was carried out with 55 SNPs, which resulted in the classification of inbreds into 4 groups based on PC1 (52%) and PC2 (11%), thus causing differentiation between the inbreds. A similar classification pattern for PCA was observed from hierarchical clustering analysis. The SNP set developed in this study has the potential for application to cultivar identification, F1 seed purity test, and marker-assisted backcross (MABC) not only for 20 elite inbreds but also for diverse resources for watermelon breeding.
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참고문헌 (25)
참고문헌 신청
Bertrand CY / 2008 / Marker-assisted selection: an approach for precision plant breeding in the twenty-first century / Philos Trans R Soc Lond B Biol Sci 363(1491):557~572
Che K / 2003 / Genetic assessment of watermelon germplasm using the AFLP technique / Science 38(1):81~84
최영미 / 2012 / EST-SSR 마커 적용을 통한 수박 F1 품종 순도 검정 / 농업생명과학연구 46(4):85~92
Edwards D / 2010 / Plant genome sequencing: applications for crop improvement / Plant Biotechnol J 8(1):2~9
Elshire RJ / 2011 / A robust, simple genotyping-bysequencing (GBS) approach for high diversity species / PLoS One 6(5):1~10
Che K / 2003 / Genetic assessment of watermelon germplasm using the AFLP technique / Science 38(1):81~84
최영미 / 2012 / EST-SSR 마커 적용을 통한 수박 F1 품종 순도 검정 / 농업생명과학연구 46(4):85~92
Edwards D / 2010 / Plant genome sequencing: applications for crop improvement / Plant Biotechnol J 8(1):2~9
Elshire RJ / 2011 / A robust, simple genotyping-bysequencing (GBS) approach for high diversity species / PLoS One 6(5):1~10
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