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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 30 - 36 (7page)
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초록· 키워드
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Brassinosteroids (BRs) are essential plant steroid hormones required for cell elongation, plant growth, development and abiotic and biotic stress tolerance. BRs are recognized by BRI1 receptor kinase that is localized in the plasma membrane, and the BRI1 protein will eventually autophosphorylate in the intracellular domain and transphosphorylate BAK1, which is a co-receptor in Arabidopsis thaliana. However, little is known of the role OsBRI1 receptor kinase plays in Oryza sativa, monocotyledonous plants, compared to that in Arabidopsis thaliana, dicotyledonous plants. As such, we have studied OsBRI1 receptor kinase in vitro and in vivo with recombinant protein and transgenic plants, whose phenotypes were also investigated. A OsBRI1 cytoplasmic domain (CD) recombinant protein was induced in BL21 (DE3) E.coli cells with IPTG, and purified to obtain OsBRI1 recombinant protein. Based on Western blot analysis with phospho-specific pTyr and pThr antibodies, OsBRI1 recombinant protein and OsBRI1-Flag protein were phosphorylated on Threonine residue(s), however, not on Tyrosine residue(s), both in vitro and in vivo. This is particularly intriguing as AtBRI1 protein was phosphorylated on both Ser/Thr and Tyr residues. Also, the OsBRI1 full-length gene was expressed in, and rescued, bri1-5 mutants, such as is seen in normal wild-type plants where AtBRI1-Flag rescues bri1-5 mutant plants. Root growth in seedlings decreased in Ws2, AtBRI1, and 3 independent OsBRI1 transgenic seedlings and had an almost complete lack of response to brassinolide in the bri1-5 mutant. In conclusion, OsBRI1, an orthologous gene of AtBRI1, can mediate normal BR signaling for plant growth and development in Arabidopsis thaliana.
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등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Clouse SD / 1996 / A brassinosteroidinsensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development / Plant Physiol 111:671~678
Clouse SD / 2011 / Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development / The Plant Cell 23:1219~1230
Gampala SS / 2007 / An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis / Developmental cell 13:177~189
Gendron JM / 2007 / Multiple mechanisms modulate brassinosteroid signaling / Current opinion in plant biology 10:436~441
Gruszka D / 2013 / The brassinosteroid signaling pathway—new key players and interconnections with other signaling networks crucial for plant development and stress tolerance / International Journal of Molecular Sciences 14:8740~8774
Clouse SD / 2011 / Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development / The Plant Cell 23:1219~1230
Gampala SS / 2007 / An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis / Developmental cell 13:177~189
Gendron JM / 2007 / Multiple mechanisms modulate brassinosteroid signaling / Current opinion in plant biology 10:436~441
Gruszka D / 2013 / The brassinosteroid signaling pathway—new key players and interconnections with other signaling networks crucial for plant development and stress tolerance / International Journal of Molecular Sciences 14:8740~8774
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