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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 359 - 366 (8page)
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초록· 키워드
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Tradescantia is a perennial plant in the family of Commelinaceae. It is known to be sensitive to radiation. In this study, Tradescantia BNL 4430 was irradiated with gamma radiation at doses of 50 to 1,000 mGy in a phytotron equipped with a 60 Co radiation source at Korea Atomic Energy Research Institute, Korea. At 13 days after irradiation, we extracted RNA from irradiated floral tissues for RNA-seq. Transcriptome assembly produced a total of 77, 326 unique transcripts. In plantlets exposed to 50, 250, 500, and 1000 mGy, the numbers of up-regulated genes with more than 2- fold of expression compared that in the control were 116, 222, 246, and 308, respectively. Most of the up-regulated genes induced by 50 mGy were heat shock proteins (HSPs) such as HSP 70, indicating that protein misfolding, aggregation, and translocation might have occurred during radiation stress. Similarly, highly up-regulated transcripts of the IQ-domain 6 were induced by 250 mGy, KAR-UP oxidoreductase 1 was induced by 500 mGy, and zinc transporter 1 precursor was induced by 1000 mGy. Reverse transcriptase (RT) PCR and quantitative real time PCR (qRT-PCR) further validated the increased mRNA expression levels of selected genes, consistent with DEG analysis results. However, 2.3 to 97- fold higher expression activities were induced by different doses of radiation based on qRT-PCR results. Results on the transcriptome of Tradescantia in response to radiation might provide unique identifiers to develop in situ monitoring kit for measuring radiation exposure around radiation facilities.
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참고문헌 (49)
참고문헌 신청
Assi NE / 1997 / Irradiation-induced changes in tomato fruit and pericarp firmness, electrolyte efflux, and cell wall enzyme activity as influenced by ripening stage / J Am Soc Hortic Sci 122:100~106
Arabi MIE / 2005 / Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation / In Vitro Cell Dev Biol Plant 41:762~764
Bhattarai KK / 2007 / The MI-1-mediated pest resistance requires Hsp90 and Sgt1 / Plant Physiol 144:312~323
Boston RS / 1996 / Molecular chaperone Hsp104—a molecular machine for protein disaggregation / J Struct Biol 156:139~148
Breiman A / 2014 / Plant Hsp90 and its co-chaperones / Curr Protein Pept Sci 15:232~244
Arabi MIE / 2005 / Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation / In Vitro Cell Dev Biol Plant 41:762~764
Bhattarai KK / 2007 / The MI-1-mediated pest resistance requires Hsp90 and Sgt1 / Plant Physiol 144:312~323
Boston RS / 1996 / Molecular chaperone Hsp104—a molecular machine for protein disaggregation / J Struct Biol 156:139~148
Breiman A / 2014 / Plant Hsp90 and its co-chaperones / Curr Protein Pept Sci 15:232~244
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