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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 71 - 76 (6page)
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The Arabidopsis SHL1 (Short Life 1) gene encodes a small nuclear protein that is critical for the proper expression of the developmental programs that are responsible for controlling plant stature, senescence, flowering and seed formation. The SHL1 contains a single PHD finger domain that works in conjunction with a bromo-adjacent homology (BAH) motif that is thought to function significantly in protein-protein interactions. The TCH4 gene of the Arabidopsis encodes a xylogluclan endotransglucosylase/hydrolase that is transcriptionally regulated by a variety of hormonal and environmental stimuli. We report here in this study that the SHL1 exhibits sequence specific DNA binding properties, recognizing a 14 bp region of the TCH4 promoter in vitro, spanning nucleotides –262 to –275 (GGAAAAAACTCCCA). Chiefly, the nuclear extracts of Arabidopsis contain a protein with similar binding properties as recombinant SHL1, which is absent in identified transgenic plants that are noted as expressing antisense SHL1 RNA. Interestingly, the SHL1 gene expression with a BL treatment in characteristically wild types of seedlings showed that the transcript level of SHL1 is significantly down regulated by the BL treatment. The SHL1 may play a subtle role in regulating the kinetics of induction of the TCH4 in response to several stimuli in vivo.
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참고문헌 (11)
참고문헌 신청
Ausubel FM / 1994 / Current Protocols in Molecular Biology / John Wiley & Sons
Beato / 1995 / Steroid hormone receptors: many actors in search of a plot / Cell 83:851~857
Bent / 1994 / RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes / Science 265:1856~1860
Clouse / 1993 / Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana / J Plant Growth Regul 12:61~66
Iliev / 2002 / Transcriptional and posttranscriptional regulation of Arabidopsis TCH4 expression by diverse stimuli. Roles of cis regions and brassinosteroids / Plant Physiol 130:770~783
Beato / 1995 / Steroid hormone receptors: many actors in search of a plot / Cell 83:851~857
Bent / 1994 / RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes / Science 265:1856~1860
Clouse / 1993 / Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana / J Plant Growth Regul 12:61~66
Iliev / 2002 / Transcriptional and posttranscriptional regulation of Arabidopsis TCH4 expression by diverse stimuli. Roles of cis regions and brassinosteroids / Plant Physiol 130:770~783
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