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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 240 - 247 (8page)
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초록· 키워드
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In this study, routinely used transformation method, which includes transferring explants onto co-cultivation medium after inoculating them with bacterial solution for a while, was compared with 3 different inoculation methods. In every 3 methods, hypocotyl explants excised from 7-day-old sterile flax seedlings having cotyledon leaves and no root system dried under air flow in sterile cabin for 35 min were inoculated with different volumes of bacterial solution at different inoculation periods. GV2260 line of Agrobacterium tumefaciens having ‘pBIN 19’ plasmid containing npt II (neomycin phosphotransferase II) gene and GUS reporter gene was used in transformation studies. After inoculation, hypocotyl segments of seedlings (0.5 cm in length) - were excised and left to co-cultivation for 2 days. Then, explants were transferred to regeneration medium supplemented with different antibiotics. The presence of npt-II and GUS genes in transformants was confirmed by PCR and GUS analysis. The highest results in all characters examined in all cultivars were obtained from the 2 inoculation method in which hypocotyls excised from seedlings inoculated with 500 μl of bacterial solution after drying in sterile cabin for 35 min were used.
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참고문헌 (36)
참고문헌 신청
Amoah BK / 2001 / Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue / J Exp Bot 52:1135~1142
Beranova M / 2008 / Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum L.) / Plant Cell Tiss Organ Cult 94:253~259
Bevan M / 1982 / Multiple transcripts of T-DNA detected in nopaline crown gall tumors / J Mol Appl Genet 1:539
Binns AN / 1988 / Cell biology of Agrobacterium infection and transformation of plants / Annu Rev Microbiol 42:575~606
Caillot S / 2009 / Influence of light intensity and selection scheme on regeneration time of transgenic flax plants / Plant Cell Rep 28:359~371
Beranova M / 2008 / Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum L.) / Plant Cell Tiss Organ Cult 94:253~259
Bevan M / 1982 / Multiple transcripts of T-DNA detected in nopaline crown gall tumors / J Mol Appl Genet 1:539
Binns AN / 1988 / Cell biology of Agrobacterium infection and transformation of plants / Annu Rev Microbiol 42:575~606
Caillot S / 2009 / Influence of light intensity and selection scheme on regeneration time of transgenic flax plants / Plant Cell Rep 28:359~371
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