지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 81 - 86 (6page)
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초록· 키워드
오류제보하기
An efficient and reproducible plant regeneration system was established using transverse thin cell layers (tTCLs) in five cultivars of Brassica juncea L. The effects of medium conditions, explant types (tTCLs of hypcotyl and cotyledonary petiole) on shoot regeneration were examined in this study. The maximum shoot regeneration frequency was obtained in Murashige and Skoog (MS) medium supplemented with 4 mg/L 6-benzylaminopurine (BA) and 0.2 mg/L 1-naphthaleneacetic acid (NAA). The hypocotyls derived tTCL explants had more shoot regeneration frequency (52%) than the cotyledonary petiole derived tTCL explants. Shoot induction was further improved by the addition of silver nitrate (AgNO3) in the regeneration medium. A significant genotypic effect was also observed between the five cultivars; Rai-5 displayed higher capacities to produce shoots than other cultivars. Regenerated shoots were rooted on MS basal medium without PGRs which induced 90% of roots. The plantlets established in greenhouse conditions with 99% survival, flowered normally and set seeds. The regenerated plants were fertile and identical to source plants.
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등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Akasada-Kennedy Y / 2005 / Efficient lant regeneration from leaves of rapeseed (Brassica napusL.): The influence of AgNO3 and genotype / Plant Cell Rep 24 : 649 ~ 654
Burnett L / 1994 / Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene
Charest PJ / 1988 / Agrobacterium-mediated transformation of thin cell layer explants from Brassica napus L / Theor Appl Genet 75 : 438 ~ 445
Chatterjee G / 1985 / Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) / Czern. Plant Cell Rep 4 : 245 ~ 247
Chi GL / 1991 / Role of ethylene on de novo shoot regeneration cotyledonary explants of Brassica campestris pekinensis (Lour) Olsson in vitro / Plant Physiol 96 : 178 ~ 183
Burnett L / 1994 / Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene
Charest PJ / 1988 / Agrobacterium-mediated transformation of thin cell layer explants from Brassica napus L / Theor Appl Genet 75 : 438 ~ 445
Chatterjee G / 1985 / Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) / Czern. Plant Cell Rep 4 : 245 ~ 247
Chi GL / 1991 / Role of ethylene on de novo shoot regeneration cotyledonary explants of Brassica campestris pekinensis (Lour) Olsson in vitro / Plant Physiol 96 : 178 ~ 183
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