지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 133 - 139 (7page)
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초록· 키워드
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The influences of ethylene inhibitors (AgNO3 and silver thiosulfate) and cytokinins (BAP and TDZ) on shoot regeneration from cotyledon and hypocotyl explants of B. napus cv. Youngsan were investigated. The presence of 50 μM Silver thiosulfate (STS) in shoot regeneration medium formed shoots at 60-68% after 3-4 weeks of culture, which was enhanced by 2-fold compared to that of Silver nitrate (AgNO3). Moreover, cotyledon explants were more regenerative than hypocotyls; shoots from cotyledon explants began to occur 4-5 days earlier than that of hypocotyl explants. TDZ at a concentration of 8-10 μM was effective for shoot regeneration, compared with BAP. Consequently, the optimal shoot regeneration response was observed in medium supplemented with 50 μM STS + 8 μM TDZ. In transmission electron microscopy (TEM) analysis, higher density of silver nanoparticles was shown to be accumulated widely inside the cell wall and plasmodesmata of regenerating leaf cultured in medium supplemented with AgNO3. By contrast, in the cell cultured in medium with STS, fine-grained deposits were partly observed in the surroundings of the cell wall.
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참고문헌 (24)
참고문헌 신청
Adams NWH / 1998 / Reactivity of Ag+ ion with thiol ligands in the presence of iron sulfide / Environ Toxicol 17 : 625 ~ 629
Apelbaum A / 1972 / Effect of ethylene on cellular differentiation in etiolated Pea seedlings / Amer J Bot 59 : 697 ~ 705
Asharani PV / 2009 / Cytotoxicity and genotoxicity of silver nanoparticles in Human cells / ACS Nano 3 : 279 ~ 290
Bano R / 2010 / Callogenesis and orgarnogenesis in three genotypes of Brassica juncea affected by explants source / Pak J Bot 42 : 3925 ~ 3932
Biesaga-Koscielniak J / 2010 / The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat / Acta Physiol Plant 32 : 1047 ~ 1053
Apelbaum A / 1972 / Effect of ethylene on cellular differentiation in etiolated Pea seedlings / Amer J Bot 59 : 697 ~ 705
Asharani PV / 2009 / Cytotoxicity and genotoxicity of silver nanoparticles in Human cells / ACS Nano 3 : 279 ~ 290
Bano R / 2010 / Callogenesis and orgarnogenesis in three genotypes of Brassica juncea affected by explants source / Pak J Bot 42 : 3925 ~ 3932
Biesaga-Koscielniak J / 2010 / The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat / Acta Physiol Plant 32 : 1047 ~ 1053
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