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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 291 - 296 (6page)
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Cyclin-dependent kinase 2 (CDK2) is a member of serine/threonine protein kinases, which initiates the principal transitions of the eukaryotic cell cycle and is a promising target for cancer therapy. The present study was designed to inhibit cdk2 gene expression to induce cell cycle arrest and cell proliferation suppression. Here, we constructed a series of RNA interference (RNAi) plasmids which can successfully express small interference RNA (siRNA) in the transfected human cells.
The results showed that the RNAi plasmids containing the coding sequences for siRNAs down-regulated the cdk2 gene expression in human cancer cells at the mRNA and the protein levels. Furthermore, we found that the cell cycle was arrested at G0G1 phases and the cell proliferation was inhibited by different siRNAs. These results demonstrate that suppression of CDK2 activity by RNAi may be an effective strategy for gene therapy in human cancers.
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참고문헌 (25)
참고문헌 신청
Bantounas, I. / 2004 / RNA interference and the use of small interfering RNA to study gene function in mammalian systems / J. Mol. Endocrinol. 33 : 545 ~ 557
Du, J. / 2004 / Critical role of cdk2 for melanoma growth linked to its melanocyte-specific transcriptional regulation by MITF / Cancer Cell 6 : 565 ~ 576
Elbashir, S. M. / 2001 / Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells / Nature 411 : 494 ~ 498
Fire, A. / 1998 / Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans / Nature 391 : 806 ~ 811
Fjose, A. / 2001 / RNA interference: mechanisms and applications / Biotechnol. Annu. Rev. 7 : 31 ~ 57
Du, J. / 2004 / Critical role of cdk2 for melanoma growth linked to its melanocyte-specific transcriptional regulation by MITF / Cancer Cell 6 : 565 ~ 576
Elbashir, S. M. / 2001 / Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells / Nature 411 : 494 ~ 498
Fire, A. / 1998 / Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans / Nature 391 : 806 ~ 811
Fjose, A. / 2001 / RNA interference: mechanisms and applications / Biotechnol. Annu. Rev. 7 : 31 ~ 57
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