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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Stroke & Neural Stem cell Laboratory in Clinical Research Institute, Department of Neurology, Seoul National University Hospital, Seoul National University, Seoul, South Korea*,Department of Neurology, Seoul National Hospital, Seoul, South Korea†Department of Neurology, Ilsan Paik Hospital, Inje University, Goyang, South Korea‡
Background: Granulocyte colony-stimulating factor (G-CSF) has neuroprotective effects against experimentalfocal cerebral ischemia. However, its neuroprotective effect on human brain is unknown. In this study we attempt-ed to determine whether G-CSF can protect the neuronal cell line derived from human cortex against in vitroischemia.Methods: Human cortical-neuroblastoma hybrid cell line (A1G11) was exposed to hypoxia with glu-cose deprivation (6 hours). To test the protective effect of G-CSF, human recombinant G-CSF (0, 5, 25, 50, or 100ng/ml) was used. Cell viability or cytotoxicity was determined by MTT or LDH assay. Flow cytometry by annex-in-V and propidium iodide (PI) and immunocytochemistry by active caspase-3 antibody were performed to detectapoptotic or viable cells. Results: Morphological changes suggestive of neuronal damage were observed follow-ing ischemia. G-CSF promoted cell survival (42% vs 32%) and decreased cytotoxicity (9% vs 18%) at a maximal-ly effective concentration of 25 ng/ml. From the flow cytometric analysis, G-CSF reduced early apoptotic (annex-in V+/PI-), and late apoptotic or necrotic (annexin V+/PI+) cells. A decrease in immunoreactivity against activecaspase-3 in the G-CSF-treated group was correlated with the neuronal protection.Conclusions: Our results pro-vide evidence that G-CSF can protect human cortical hybrid neurons from apoptotic or necrotic cell death after anischemic insult.
대한뇌졸중학회지제6권제1호2004년5월
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