Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy

윤웅배; 김현진; 김광기; 최용두; 장희진; 손대경

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자료유형: 학술저널

저자정보: 윤웅배 김현진 김광기 최용두 장희진 손대경

저널정보: 대한의료정보학회 Healthcare Informatics Research Healthcare Informatics Research 제22권 제3호

발행연도: 2016.1

수록면: 238 - 242 (5page)

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연구결과

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Objectives: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. Methods: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. Results: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. Conclusions: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.

#Confocal Laser Scanning Microscopy #Computer-Assisted Image Processing #Fluorescence #Histological Labeling #Hematoxylin

참고문헌 (12)

참고문헌 신청

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Ferreiro JA / 1995 / Accuracy of frozen section diagnosis in surgical pathology: review of a 1-year experience with 24,880 cases at Mayo Clinic Rochester / Mayo Clin Proc 70(12):1137~1141

Smith GJ / 1991 / Application of confocal scanning laser microscopy in experimental pathology / J Electron Microsc Tech 18(1):38~49

Fink-Puches R / 1995 / Confocal laser scanning microscopy: a new optical microscopic technique for applications in pathology and dermatology / J Cutan Pathol 22(3):252~259

Ragazzi M / 2014 / Fluorescence confocal microscopy for pathologists / Mod Pathol 27(3):460~471

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