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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국정보기술학회 JOURNAL OF ADVANCED INFORMATION TECHNOLOGY AND CONVERGENCE Journal of Advanced Information Technology and Convergence Vol.9 No.1
- 발행연도
- 2019.7
- 수록면
- 103 - 113 (11page)
- DOI
- 10.14801/jaitc.2019.9.1.103
이용수
초록· 키워드
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Motivation: Polymerized actin-based cytoskeletal structures are crucial in shape, dynamics, and resilience of a cell. For example, dynamical actin-containing ruffles are located at leading edges of cells and have a significant impact on cell motility. Other filamentous actin (F-actin) bundles, called stress fibers, are essential in cell attachment and detachment. For this reason, their mechanistic understanding provides crucial information to solve practical problems related to cell interactions with materials in tissue engineering. Detecting and counting actin-based structures in a cellular ensemble is a fundamental first step. In this research, we suggest a new method to characterize F-actin wrapping fibers from confocal fluorescence image stacks. As fluorescently labeled F-actin often envelope the fibers, we first propose to segment these fibers by diminishing an energy based on maximum flow and minimum cut algorithm. The actual actin is detected through the use of bilateral filtering followed by a thresholding step. Later, concave actin bundles are detected through a graph-based procedure that actually determines if the considered actin filament is enclosing the fiber.
목차
Abstract
1. Introduction
2. Methods
3. Results
4. Discussion and future research
References
참고문헌 (27)
참고문헌 신청
L. Moldovan / 2000 / Redox changes of cultured endothelial cells and actin dynamics. Moldovan / Circulation Research 86(5):549~557
Hongsheng Li / 2011 / Actin Filament Segmentation Using Dynamic Programming / Int'l Conf. Information Processing in Medical Imaging (IPMI) :411~423
Hongsheng Li / 2010 / Actin Filament Segmentation Using Spatiotemporal Active-Surface and Active-Contour Models / Int'l Conf. Medical Image Computing and Computer Assisted Intervention (MICCAI)
Hongsheng Li / 2009 / Actin Filament Tracking Based on Particle Filters and Stretching Open Active Contour Models / Int'l Conf. Medical Image Computing and Computer Assisted Intervention (MICCAI)
Hongsheng Li / 2009 / Automated Actin Filament Segmentation, Tracking, and Tip Elongation Measurements based on Open Active Contour Models / IEEE Int'l Symp. Biomedical Imaging: From Nano to Macro (ISBI)
Hongsheng Li / 2011 / Actin Filament Segmentation Using Dynamic Programming / Int'l Conf. Information Processing in Medical Imaging (IPMI) :411~423
Hongsheng Li / 2010 / Actin Filament Segmentation Using Spatiotemporal Active-Surface and Active-Contour Models / Int'l Conf. Medical Image Computing and Computer Assisted Intervention (MICCAI)
Hongsheng Li / 2009 / Actin Filament Tracking Based on Particle Filters and Stretching Open Active Contour Models / Int'l Conf. Medical Image Computing and Computer Assisted Intervention (MICCAI)
Hongsheng Li / 2009 / Automated Actin Filament Segmentation, Tracking, and Tip Elongation Measurements based on Open Active Contour Models / IEEE Int'l Symp. Biomedical Imaging: From Nano to Macro (ISBI)
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UCI(KEPA) : I410-ECN-0101-2019-004-000944899