지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 470 - 475 (6page)
이용수
초록· 키워드
오류제보하기
Background: It was previously found that Korean Red Ginseng water extract (KRGE) inhibits the histamine-induced itch signaling pathway in peripheral sensory neurons. Thus, in the present study, we investigated whether KRGE inhibited another distinctive itch pathway induced by chloroquine (CQ); a representative histamine-independent pathway mediated by MrgprA3 and TRPA1.
Methods: Intracellular calcium changes were measured by the calcium imaging technique in the HEK293T cells transfected with both MrgprA3 and TRPA1 (“MrgprA3/TRPA1”), and in primary culture of mouse dorsal root ganglia (DRGs). Mouse scratching behavior tests were performed to verify proposed antipruritic effects of KRGE and ginsenoside Rg3.
Results: CQ-induced Ca<SUP>2+</SUP> influxwas strongly inhibited by KRGE (10 mg/mL) in MrgprA3/TRPA1, and notably ginsenoside Rg3 dose-dependently suppressed CQ-induced Ca<SUP>2+</SUP> influx in MrgprA3/TRPA1. Moreover, both KRGE (10 mg/mL) and Rg3 (100 mM) suppressed CQ-induced Ca<SUP>2+</SUP> influx in primary culture of mouse DRGs, indicating that the inhibitory effect of KRGE was functional in peripheral sensory neurons. In vivo tests revealed that not only KRGE (100 mg) suppressed CQ-induced scratching in mice [bouts of scratching: 274.0±51.47 (control) vs. 104.7±17.39 (KRGE)], but also Rg3 (1.5 mg) oral administration significantly reduced CQ-induced scratching as well [bouts of scratching: 216.8±33.73 (control) vs.115.7±20.94 (Rg3)].
Conclusion: The present study verified that KRGE and Rg3 have a strong antipruritic effect against CQinduced itch. Thus, KRGE is as a promising antipruritic agent that blocks both histamine-dependent and -independent itch at peripheral sensory neuronal levels.
Methods: Intracellular calcium changes were measured by the calcium imaging technique in the HEK293T cells transfected with both MrgprA3 and TRPA1 (“MrgprA3/TRPA1”), and in primary culture of mouse dorsal root ganglia (DRGs). Mouse scratching behavior tests were performed to verify proposed antipruritic effects of KRGE and ginsenoside Rg3.
Results: CQ-induced Ca<SUP>2+</SUP> influxwas strongly inhibited by KRGE (10 mg/mL) in MrgprA3/TRPA1, and notably ginsenoside Rg3 dose-dependently suppressed CQ-induced Ca<SUP>2+</SUP> influx in MrgprA3/TRPA1. Moreover, both KRGE (10 mg/mL) and Rg3 (100 mM) suppressed CQ-induced Ca<SUP>2+</SUP> influx in primary culture of mouse DRGs, indicating that the inhibitory effect of KRGE was functional in peripheral sensory neurons. In vivo tests revealed that not only KRGE (100 mg) suppressed CQ-induced scratching in mice [bouts of scratching: 274.0±51.47 (control) vs. 104.7±17.39 (KRGE)], but also Rg3 (1.5 mg) oral administration significantly reduced CQ-induced scratching as well [bouts of scratching: 216.8±33.73 (control) vs.115.7±20.94 (Rg3)].
Conclusion: The present study verified that KRGE and Rg3 have a strong antipruritic effect against CQinduced itch. Thus, KRGE is as a promising antipruritic agent that blocks both histamine-dependent and -independent itch at peripheral sensory neuronal levels.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (39)
참고문헌 신청
[학술지(정기간행물)] - Kwang-Soo Baek / 2016 / In vitro and in vivo anti-inflammatory activities of Korean Red Ginseng-derived components / Journal of Ginseng Research 40 (4) : 437 ~ 444
[학술지(정기간행물)] - 임경택 / 2016 / Ginseng, the natural effectual antiviral: Protective effects of Korean Red Ginseng against viral infection / Journal of Ginseng Research 40 (4) : 309 ~ 314
[학술지(정기간행물)] - 이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38 (3) : 161 ~ 166
[학술지(정기간행물)] - Trinh HT / 2008 / Evaluation of antipruritic effects of red ginseng and its ingredients in mice / Planta Med 74 : 210 ~ 214
[학술지(정기간행물)] - Samukawa K / 2012 / Red ginseng inhibits scratching behavior associated with atopic dermatitis in experimental animal models / J Pharmacol Sci 118 : 391 ~ 400
[학술지(정기간행물)] - 임경택 / 2016 / Ginseng, the natural effectual antiviral: Protective effects of Korean Red Ginseng against viral infection / Journal of Ginseng Research 40 (4) : 309 ~ 314
[학술지(정기간행물)] - 이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38 (3) : 161 ~ 166
[학술지(정기간행물)] - Trinh HT / 2008 / Evaluation of antipruritic effects of red ginseng and its ingredients in mice / Planta Med 74 : 210 ~ 214
[학술지(정기간행물)] - Samukawa K / 2012 / Red ginseng inhibits scratching behavior associated with atopic dermatitis in experimental animal models / J Pharmacol Sci 118 : 391 ~ 400
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