지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 437 - 444 (8page)
이용수
초록· 키워드
오류제보하기
Background: Although Korean Red Ginseng (KRG) has been traditionally used for a long time, its anti-inflammatory role and underlying molecular and cellular mechanisms have been poorly understood.In this study, the anti-inflammatory roles of KRG-derived components, namely, water extract (KRG-WE),saponin fraction (KRG-SF), and nonsaponin fraction (KRG-NSF), were investigated.
Methods: To check saponin levels in the test fractions, KRG-WE, KRG-NSF, and KRG-SF were analyzed using high-performance liquid chromatography. The anti-inflammatory roles and underlying cellular and molecular mechanisms of these components were investigated using a macrophage-like cell line (RAW264.7 cells) and an acute gastritis model in mice.
Results: Of the tested fractions, KGR-SF (but not KRG-NSF and KRG-WE) markedly inhibited the viability of RAW264.7 cells, and splenocytes at more than 500 ㎍/mL significantly suppressed NO production at 100 ㎍/mL, diminished mRNA expression of inflammatory genes such as inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interferon-β at 200 ㎍/mL, and completely blocked phagocytic uptake by RAW264.7 cells. All three fractions suppressed luciferase activity triggered by interferon regulatory factor 3 (IRF3), but not that triggered by activator protein-1 and nuclear factor-kappa B. Phospho-IRF3 and phospho-TBK1 were simultaneously decreased in KRG-SF. Interestingly, all these fractions, when orally administered, clearly ameliorated the symptoms of gastric ulcer in HCl/ethanol-induced gastritis mice.
Conclusion: These results suggest that KRG-WE, KRG-NSF, and KRG-SF might have anti-inflammatory properties, mostly because of the suppression of the IRF3 pathway.
Methods: To check saponin levels in the test fractions, KRG-WE, KRG-NSF, and KRG-SF were analyzed using high-performance liquid chromatography. The anti-inflammatory roles and underlying cellular and molecular mechanisms of these components were investigated using a macrophage-like cell line (RAW264.7 cells) and an acute gastritis model in mice.
Results: Of the tested fractions, KGR-SF (but not KRG-NSF and KRG-WE) markedly inhibited the viability of RAW264.7 cells, and splenocytes at more than 500 ㎍/mL significantly suppressed NO production at 100 ㎍/mL, diminished mRNA expression of inflammatory genes such as inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interferon-β at 200 ㎍/mL, and completely blocked phagocytic uptake by RAW264.7 cells. All three fractions suppressed luciferase activity triggered by interferon regulatory factor 3 (IRF3), but not that triggered by activator protein-1 and nuclear factor-kappa B. Phospho-IRF3 and phospho-TBK1 were simultaneously decreased in KRG-SF. Interestingly, all these fractions, when orally administered, clearly ameliorated the symptoms of gastric ulcer in HCl/ethanol-induced gastritis mice.
Conclusion: These results suggest that KRG-WE, KRG-NSF, and KRG-SF might have anti-inflammatory properties, mostly because of the suppression of the IRF3 pathway.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (33)
참고문헌 신청
[학술지(정기간행물)] - Zou J / 2015 / Lipopolysaccharide-induced tumor necrosis factor-alpha factor enhances inflammation and is associated with cancer (Review) / Mol Med Rep 12 : 6399 ~ 6404
[학술지(정기간행물)] - Ferrero-Miliani L / 2007 / Chronic inflammation:importance of NOD2 and NALP3 in interleukin-1beta generation / Clin Exp Immunol 147 : 227 ~ 235
[학술지(정기간행물)] - Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm
[학술지(정기간행물)] - Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm
[학술지(정기간행물)] - 김준호 / 2015 / Fisetin Suppresses Macrophage-Mediated Inflammatory Responses by Blockade of Src and Syk / Biomolecules & Therapeutics 23 (5) : 414 ~ 420
[학술지(정기간행물)] - Ferrero-Miliani L / 2007 / Chronic inflammation:importance of NOD2 and NALP3 in interleukin-1beta generation / Clin Exp Immunol 147 : 227 ~ 235
[학술지(정기간행물)] - Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm
[학술지(정기간행물)] - Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm
[학술지(정기간행물)] - 김준호 / 2015 / Fisetin Suppresses Macrophage-Mediated Inflammatory Responses by Blockade of Src and Syk / Biomolecules & Therapeutics 23 (5) : 414 ~ 420
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UCI(KEPA) : I410-ECN-0101-2018-524-001598587