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자료유형
학술저널
저자정보
송인규 (강원대학교) 최병현 (강원대학교) 홍억기 (강원대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.33 No.2(Wn.165)
발행연도
2018.6
수록면
110 - 117 (8page)
DOI
10.7841/ksbbj.2018.33.2.110

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This study was carried out to investigate the optimum liquid culture conditions for the mycelium and the polysaccharide production on liquid culture of Inonotus obliquus. In flask culture, the production of maximum mycelium and polysaccharide were 9.23 g/L and 1.85 g/L, respectively. In bioreactor, with the culture conditions of agitation rate 200 rpm and aeration rate 1 vvm, the production of mycelium and polysaccharide were 14.17 g/L and 2.81 g/L at the 8<SUP>th</SUP> day of cultivation, respectively. And the cultivation was operated with 300 rpm and 1 vvm up to the 4<SUP>th</SUP> day of cultivation, since then, 200 rpm and 1 vvm, resulting in that the production of mycelium was 15.3 g/L at the 9th day and the polysaccharide production was 2.93 g/L at the 6th day of the cultivation. Furthermore, with the same agitation and aeration rates as the above during the cultivation, the addition of glucose being 20g/L of the final concentration was intermittently accomplished in order to increase the mycelium and the polysaccharide production. As a result, the addition of glucose at the 3<SUP>rd</SUP> day of cultivation resulted in that the maximum mycelium and the polysaccharide production were 17.1 g/L and 3.4 g/L at the 7<SUP>th</SUP> day of cultivation, respectively, indicating that the cultivation period was 4 days shorter than the cultivation of 11 days.

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Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
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UCI(KEPA) : I410-ECN-0101-2018-470-003109998