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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.44 No.4
- 발행연도
- 2017.12
- 수록면
- 586 - 594 (9page)
이용수
초록· 키워드
오류제보하기
Mitochondrial activity affects skeletal muscle energy metabolism and phenotype. To address whether mitochondrial activity can modulate muscle phenotype in vitro, protein expression of myosin heavy chain (MyHC) in C2C12 muscle cell lines was investigated after treated with antimycin A, an inhibitor of oxidative phosphorylation in mitochondria. Fully differentiated C2C12 myotubes were administrated with different concentration of antimycin A including 0, 100, 200, 500, 700, and 1000 ng/mL. After 72 h treatment, myosin heavy chain isoform expression and related enzyme activity (lactate dehydrogenase; LDH and creatine kinase) were analyzed. Administration of antimycin A changed expression of MyHC in C2C12 myotubes showing a shift from slow to fast twitching muscle type. Protein expression of MyHC type 2b (fast twitching muscle type) was decreased (P < 0.05) by antimycin A treatment (500, 700, and 1000 ng/mL) when compared with control group. Administration of antimycin A (1000 ng/mL), however, decreased (P < 0.05) MyHC type I (slow twitching muscle type). Interestingly, LDH activity was increased (P < 0.05) by antimycin A treatment. Results from our current study proposed a possibility that skeletal muscle phenotype, including MyHC and LDH activity, can be shifted from slow to fast twitching type by inhibiting the mitochondrial activity in C2C12 myotubes.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (21)
참고문헌 신청
[학술지(정기간행물)] - Booth RF / 1978 / Studies on the mitochondrially bound form of rat brain creatine kinase / Biochemistry Journal 170 : 145 ~ 151
[학술지(정기간행물)] - Dickman KG / 1990 / Differential effects of respiratory inhibitors on glycolysis in proximal tubules / American Journal of Physiology - Renal Physiology 258 : F1608 ~ F1615
[학술지(정기간행물)] - Gunawan AM / 2007 / Contractile protein content reflects myosin heavy-chain isoform gene expression / Journal Animal Science 85 : 1247 ~ 1256
[학술지(정기간행물)] - Han YH / 2008 / Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells / Oncology Reports 20 : 689 ~ 693
[학술지(정기간행물)] - Handschin C / 2007 / Skeletal muscle fiber-type switching, exercise intolerance, and myopathy in PGC-1alpha muscle-specific knock-out animals / Journal Biological Chemistry 282 : 30014 ~ 30021
[학술지(정기간행물)] - Dickman KG / 1990 / Differential effects of respiratory inhibitors on glycolysis in proximal tubules / American Journal of Physiology - Renal Physiology 258 : F1608 ~ F1615
[학술지(정기간행물)] - Gunawan AM / 2007 / Contractile protein content reflects myosin heavy-chain isoform gene expression / Journal Animal Science 85 : 1247 ~ 1256
[학술지(정기간행물)] - Han YH / 2008 / Antimycin A as a mitochondrial electron transport inhibitor prevents the growth of human lung cancer A549 cells / Oncology Reports 20 : 689 ~ 693
[학술지(정기간행물)] - Handschin C / 2007 / Skeletal muscle fiber-type switching, exercise intolerance, and myopathy in PGC-1alpha muscle-specific knock-out animals / Journal Biological Chemistry 282 : 30014 ~ 30021
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UCI(KEPA) : I410-ECN-0101-2018-520-001702218