지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 328 - 334 (7page)
- DOI
- 10.7841/ksbbj.2017.32.4.328
이용수
초록· 키워드
오류제보하기
In this study, Ligustrum japonicum fructus was studied for its ability to inhibit MMP-2 and -9 using gelatin zymography and RT-PCR in Phorbol 12-myristate 13-acetate (PMA)-induced HT1080 fibrosarcoma cells. Ligustrum japonicum fructus was extracted twice with dichloromethane (CH₂Cl₂) and methanol (MeOH) in turn. The combined crude extracts (CH₂Cl₂ and MeOH) of Ligustrum japonicum fructus showed a significant inhibitory effect on MMP-2 and -9 activities in gelatin zymography. The combined crude extracts were partitioned between CH₂Cl₂ and water. The organic layer was further partitioned between 85% aq.MeOH and n-hexane,
and then the aqueous layer was fractionated with n-butanol (n-BuOH) and water, successively. Among all solvent fractions, n-BuOH and 85% aq.MeOH fractions showed the potent inhibitory effect on MMP-2 and -9 in gelatin zymography. In PMA-treated HT-1080 cells, mRNA expression of MMP-2/-9 was also greatly inhibited in the n-BuOH and 85% aq.MeOH fractions. In conclusion, these findings suggested that Ligustrum japonicum fructus can be used as a potential source of promising anti-invasive agent.
and then the aqueous layer was fractionated with n-butanol (n-BuOH) and water, successively. Among all solvent fractions, n-BuOH and 85% aq.MeOH fractions showed the potent inhibitory effect on MMP-2 and -9 in gelatin zymography. In PMA-treated HT-1080 cells, mRNA expression of MMP-2/-9 was also greatly inhibited in the n-BuOH and 85% aq.MeOH fractions. In conclusion, these findings suggested that Ligustrum japonicum fructus can be used as a potential source of promising anti-invasive agent.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (21)
참고문헌 신청
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Westermarck, J. and V. M. Kahari (1999) Regulation of matrix metalloproteinase expression in tumor invasion. FASEB J. 13: 781- 792.
Kieseier, B. C., Seifert, T., Giovannoni G., and H. P. Hartung (1999) Matrix metalloproteinases in inflammatory demyelination. Neurology 53: 20-25.
Back, M., Ketelhuth, D. F., and S. Agewall (2010) Matrix metalloproteinases in atherothrombosis. Prog. Cardiovasc. Dis. 52: 410- 428.
Hadler-Olsen, E., Fadnes, B., Sylte, I., Uhlin-Hansen, L., and J. O. Winberg (2011) Regulation of matrix metalloproteinases in health and disease. FEBS J. 278: 28-45.
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