First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal

Haroon Ahmed; Sérgio Ramalho Sousa; Sami Simsek; Sofia Anastácio; Seyma Gunyaktilinc

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자료유형: 학술저널

저자정보: Haroon Ahmed (COMSATS Institute of Information Technology) Sérgio Ramalho Sousa (Vasco da Gama University School) Sami Simsek (University of Firat) Sofia Anastácio (Vasco da Gama University School) Seyma Gunyaktilinc (University of Firat)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.6

발행연도: 2017.12

수록면: 653 - 658 (6page)

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Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

#Hypoderma actaeon #cattle #red deer #phylogenetic analysis #PCR-RFLP #Portugal

참고문헌 (27)

참고문헌 신청

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[단행본] - Zumpt F / 1965 / Myiasis in Man and Animals in the Old World / Butterworths : 205 ~ 214

[단행본] - Brauer FM / 1863 / Monographie der Oestriden / Druck Von Carl Unberreuter : 291 ~

[학술지(정기간행물)] - Sugar L / 1976 / On the incidence of larvae of Hypodermatidae in the games and wild rodents of Hungary / Parasit Hung 9 : 85 ~ 96

[학술지(정기간행물)] - Maes S / 2000 / Deer Myiasis in France / COST Action 833 : 181 ~ 186

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