지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 386 - 391 (6page)
이용수
초록· 키워드
오류제보하기
Background: Korean Red Ginseng (KRG) is an ethnopharmacological plant that is traditionally used to improve the body’s immune functions and ameliorate the symptoms of various diseases. However, the antitumorigenic effects of KRG and its underlying molecular and cellular mechanisms are not fully understood in terms of its individual components. In this study, in vitro and in vivo antitumorigenic activities of KRG were explored in water extract (WE), saponin fraction (SF), and nonsaponin fraction (NSF).
Methods: In vitro antitumorigenic activities of WE, SF, and NSF of KRG were investigated in the C6 glioma cell line using cytotoxicity, migration, and proliferation assays. The underlying molecular mechanisms of KRG fractions were determined by examining the signaling cascades of apoptotic cell death by semiquantitative reverse transcriptase polymerase chain reaction and Western blot analysis. The in vivo antitumorigenic activities of WE, SF, and NSF were investigated in a xenograft mouse model.
Results: SF induced apoptotic death of C6 glioma cells and suppressed migration and proliferation of C6 glioma cells, whereas WE and NSF neither induced apoptosis nor suppressed migration of C6 glioma cells. SF downregulated the expression of the anti-apoptotic gene B-cell lymphoma-2 (Bcl-2) and upregulated the expression of the pro-apoptotic gene Bcl-2-associated X protein (BAX) in C6 glioma cells but had no effect on the expression of the p53 tumor-suppressor gene. Moreover, SF treatment resulted in activation of caspase-3 as evidenced by increased levels of cleaved caspase-3. Finally, WE, SF, and NSF exhibited in vivo antitumorigenic activities in the xenograft mouse model by suppressing the growth of grafted CT-26 carcinoma cells without decreasing the animal body weight.
Conclusion: These results suggest that WE, SF, and NSF of KRG are able to suppress tumor growth via different molecular and cellular mechanisms, including induction of apoptosis and activation of immune cells.
Methods: In vitro antitumorigenic activities of WE, SF, and NSF of KRG were investigated in the C6 glioma cell line using cytotoxicity, migration, and proliferation assays. The underlying molecular mechanisms of KRG fractions were determined by examining the signaling cascades of apoptotic cell death by semiquantitative reverse transcriptase polymerase chain reaction and Western blot analysis. The in vivo antitumorigenic activities of WE, SF, and NSF were investigated in a xenograft mouse model.
Results: SF induced apoptotic death of C6 glioma cells and suppressed migration and proliferation of C6 glioma cells, whereas WE and NSF neither induced apoptosis nor suppressed migration of C6 glioma cells. SF downregulated the expression of the anti-apoptotic gene B-cell lymphoma-2 (Bcl-2) and upregulated the expression of the pro-apoptotic gene Bcl-2-associated X protein (BAX) in C6 glioma cells but had no effect on the expression of the p53 tumor-suppressor gene. Moreover, SF treatment resulted in activation of caspase-3 as evidenced by increased levels of cleaved caspase-3. Finally, WE, SF, and NSF exhibited in vivo antitumorigenic activities in the xenograft mouse model by suppressing the growth of grafted CT-26 carcinoma cells without decreasing the animal body weight.
Conclusion: These results suggest that WE, SF, and NSF of KRG are able to suppress tumor growth via different molecular and cellular mechanisms, including induction of apoptosis and activation of immune cells.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Rhee MY / 2014 / Blood pressure lowering effect of Korea ginseng derived ginseol K-g1 / Am J Chin Med 42 : 605 ~ 618
[학술지(정기간행물)] - Wang CZ / 2007 / Commonly used antioxidant botanicals : active constituents and their potential role in cardiovascular illness / Am J Chin Med 35 : 543 ~ 558
[학술지(정기간행물)] - Leggett S / 2015 / The impact of complementary and alternative medicines on cancer symptoms, treatment side effects, quality of life, and survival in women with breast cancerea systematic review / Nutr Cancer 67 : 373 ~ 391
[학술지(정기간행물)] - Yong-seok Han / 2015 / Antitumor Effects of Fucoidan on Human Colon Cancer Cells via Activation of Akt Signaling / Biomolecules & Therapeutics 23 (3) : 225 ~ 232
[학술지(정기간행물)] - Ang-Lee MK / 2001 / Herbal medicines and perioperative care / JAMA 286 : 208 ~ 216
[학술지(정기간행물)] - Wang CZ / 2007 / Commonly used antioxidant botanicals : active constituents and their potential role in cardiovascular illness / Am J Chin Med 35 : 543 ~ 558
[학술지(정기간행물)] - Leggett S / 2015 / The impact of complementary and alternative medicines on cancer symptoms, treatment side effects, quality of life, and survival in women with breast cancerea systematic review / Nutr Cancer 67 : 373 ~ 391
[학술지(정기간행물)] - Yong-seok Han / 2015 / Antitumor Effects of Fucoidan on Human Colon Cancer Cells via Activation of Akt Signaling / Biomolecules & Therapeutics 23 (3) : 225 ~ 232
[학술지(정기간행물)] - Ang-Lee MK / 2001 / Herbal medicines and perioperative care / JAMA 286 : 208 ~ 216
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UCI(KEPA) : I410-ECN-0101-2018-524-001599115