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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 240 - 246 (7page)
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초록· 키워드
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Background: Korean Red Ginseng (KRG) is a traditional herbal medicine made by steaming and drying fresh ginseng. It strengthens the endocrine and immune systems to ameliorate various inflammatory responses. The cyclooxygenase-2 (COX-2)/prostaglandin E2 pathway has important implications for inflammation responses and tumorigenesis. Peroxisome proliferator-activated receptor gamma (PPARg) is a transcription factor that regulates not only adipogenesis and lipid homeostasis, but also angiogenesis and inflammatory responses.
Methods: The effects of the KRG on inhibition of hypoxia-induced COX-2 via PPARg in A549 cells were determined by luciferase assay,Western blot, and/or quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The antimigration and invasive effects of KRG were evaluated on A549 cells using migration and matrigel invasion assays.
Results and conclusion: We previously reported that hypoxia-induced COX-2 protein and mRNA levels were suppressed by KRG. This study examines the possibility of PPARg as a cellular target of KRG for the suppression of hypoxia-induced COX-2. PPARg protein levels and PPARg-responsive element (PPRE)-driven reporter activities were increased by KRG. Reduction of hypoxia-induced COX-2 by KRG was abolished by the PPARg inhibitor GW9662. In addition, the inhibition of PPARg abolished the effect of KRG on hypoxia-induced cell migration and invasion.
Discussion: Our results show that KRG inhibition of hypoxia-induced COX-2 expression and cell invasion is dependent on PPARg activation, supporting the therapeutic potential for suppression of inflammation under hypoxia. Further studies are required to demonstrate whether KRG activates directly PPARg and to identify the constituents responsible for this activity.
Methods: The effects of the KRG on inhibition of hypoxia-induced COX-2 via PPARg in A549 cells were determined by luciferase assay,Western blot, and/or quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The antimigration and invasive effects of KRG were evaluated on A549 cells using migration and matrigel invasion assays.
Results and conclusion: We previously reported that hypoxia-induced COX-2 protein and mRNA levels were suppressed by KRG. This study examines the possibility of PPARg as a cellular target of KRG for the suppression of hypoxia-induced COX-2. PPARg protein levels and PPARg-responsive element (PPRE)-driven reporter activities were increased by KRG. Reduction of hypoxia-induced COX-2 by KRG was abolished by the PPARg inhibitor GW9662. In addition, the inhibition of PPARg abolished the effect of KRG on hypoxia-induced cell migration and invasion.
Discussion: Our results show that KRG inhibition of hypoxia-induced COX-2 expression and cell invasion is dependent on PPARg activation, supporting the therapeutic potential for suppression of inflammation under hypoxia. Further studies are required to demonstrate whether KRG activates directly PPARg and to identify the constituents responsible for this activity.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (46)
참고문헌 신청
[학술지(정기간행물)] - Qi LW / 2011 / Ginsenosides from American ginseng : chemical and pharmacological diversity / Phytochemistry 72 : 689 ~ 699
[학술지(정기간행물)] - Bhattacharya SK / 1991 / Anxiolytic activity of Panax ginseng roots : an experimental study / J Ethnopharmacol 34 : 84 ~ 92
[학술지(정기간행물)] - Jin Y / 2008 / American ginseng suppresses inflammation and DNA damage associated with mouse colitis / Carcinogenesis 29 : 2351 ~ 2359
[학술지(정기간행물)] - Park JS / 2012 / Antiinflammatory mechanism of compound K in activated microglia and its neuroprotective effect on experimental stroke in mice / J Pharmacol Exp Ther 341 : 59 ~ 67
[학술지(정기간행물)] - Yang Y / 2014 / ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract / J Ethnopharmacol 154 : 218 ~ 228
[학술지(정기간행물)] - Bhattacharya SK / 1991 / Anxiolytic activity of Panax ginseng roots : an experimental study / J Ethnopharmacol 34 : 84 ~ 92
[학술지(정기간행물)] - Jin Y / 2008 / American ginseng suppresses inflammation and DNA damage associated with mouse colitis / Carcinogenesis 29 : 2351 ~ 2359
[학술지(정기간행물)] - Park JS / 2012 / Antiinflammatory mechanism of compound K in activated microglia and its neuroprotective effect on experimental stroke in mice / J Pharmacol Exp Ther 341 : 59 ~ 67
[학술지(정기간행물)] - Yang Y / 2014 / ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract / J Ethnopharmacol 154 : 218 ~ 228
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UCI(KEPA) : I410-ECN-0101-2018-524-001598921