지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 141 - 150 (10page)
이용수
초록· 키워드
오류제보하기
Background: Adipocyte-macrophage communication plays a critical role regulating white adipose tissue (WAT) inflammatory gene expression. Because WAT inflammation contributes to the development of metabolic diseases, there is significant interest in understanding how exogenous compounds regulate the adipocyte-macrophage crosstalk. An aqueous (AQ) extract of North American (NA) ginseng (Panax quinquefolius) was previously shown to have strong inflammo-regulatory properties in adipocytes. This study examined whether different ginseng extracts influence adipocyte-macrophage crosstalk, as well as WAT inflammatory gene expression.
Methods: The effects of AQ and ethanol (EtOH) ginseng extracts (5 μg/mL) on adipocyte and macrophage inflammatory gene expression were studied in 3T3-L1 and RAW264.7 cells, respectively, using real-time reverse transcription polymerase chain reaction. Adipose tissue organ culture was also used to examine the effects of ginseng extracts on epididymal WAT (EWAT) and inguinal subcutaneous WAT (SWAT) inflammatory gene expression.
Results: The AQ extract caused significant increases in the expression of common inflammatory genes (e.g., Mcp1, Ccl5, Tnf-α, Nos2) in both cell types. Culturing adipocytes in media from macrophages treated with the AQ extract, and vice versa, also induced inflammatory gene expression. Adipocyte Ppar-γ expression was reduced with the AQ extract. The AQ extract strongly induced inflammatory gene expression in EWAT, but not in SWAT. The EtOH extract had no effect on inflammatory gene expression in either both cell types or WAT.
Conclusion: These findings provide important new insights into the inflammo-regulatory role of NA ginseng in WAT.
Methods: The effects of AQ and ethanol (EtOH) ginseng extracts (5 μg/mL) on adipocyte and macrophage inflammatory gene expression were studied in 3T3-L1 and RAW264.7 cells, respectively, using real-time reverse transcription polymerase chain reaction. Adipose tissue organ culture was also used to examine the effects of ginseng extracts on epididymal WAT (EWAT) and inguinal subcutaneous WAT (SWAT) inflammatory gene expression.
Results: The AQ extract caused significant increases in the expression of common inflammatory genes (e.g., Mcp1, Ccl5, Tnf-α, Nos2) in both cell types. Culturing adipocytes in media from macrophages treated with the AQ extract, and vice versa, also induced inflammatory gene expression. Adipocyte Ppar-γ expression was reduced with the AQ extract. The AQ extract strongly induced inflammatory gene expression in EWAT, but not in SWAT. The EtOH extract had no effect on inflammatory gene expression in either both cell types or WAT.
Conclusion: These findings provide important new insights into the inflammo-regulatory role of NA ginseng in WAT.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - Rosen ED / 2014 / What we talk about when we talk about fat / Cell 156 : 20 ~ 44
[학술지(정기간행물)] - Weisberg SP / 2003 / Obesity is associated with macrophage accumulation in adipose tissue / J Clin Invest 112 : 1796 ~ 1808
[학술지(정기간행물)] - Siriwardhana N / 2012 / n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-kB-dependent mechanisms / J Nutr Biochem 23 : 1661 ~ 1667
[학술지(정기간행물)] - Barnes PM / 2008 / Complementary and alternative medicine use among adults and children: United States, 2007 / Natl Health Stat Report 12 : 1 ~ 23
[학술지(정기간행물)] - Wilson SA / 2013 / Unraveling the adipocyte inflammomodulatory pathways activated by North American ginseng / Int J Obes (Lond) 37 : 350 ~ 356
[학술지(정기간행물)] - Weisberg SP / 2003 / Obesity is associated with macrophage accumulation in adipose tissue / J Clin Invest 112 : 1796 ~ 1808
[학술지(정기간행물)] - Siriwardhana N / 2012 / n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-kB-dependent mechanisms / J Nutr Biochem 23 : 1661 ~ 1667
[학술지(정기간행물)] - Barnes PM / 2008 / Complementary and alternative medicine use among adults and children: United States, 2007 / Natl Health Stat Report 12 : 1 ~ 23
[학술지(정기간행물)] - Wilson SA / 2013 / Unraveling the adipocyte inflammomodulatory pathways activated by North American ginseng / Int J Obes (Lond) 37 : 350 ~ 356
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UCI(KEPA) : I410-ECN-0101-2018-524-001598214