The optimal duration of ischemic preconditioning for renal ischemia-reperfusion injury in mice

Hyun Su Choi; Jeong Kye Hwang; Jeong Goo Kim; Hyeon Seok Hwang; Sang Ju Lee; Yoon kyung Chang; Ji Il Kim; In Sung Moon

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자료유형: 학술저널

저자정보: Hyun Su Choi (Daejeon St. Mary’s Hospital) Jeong Kye Hwang (Daejeon St. Mary’s Hospital) Jeong Goo Kim (Daejeon St. Mary’s Hospital) Hyeon Seok Hwang (Daejeon St. Mary’s Hospital) Sang Ju Lee (Daejeon St. Mary’s Hospital) Yoon kyung Chang (Daejeon St. Mary’s Hospital) Ji Il Kim (Uijeongbu St. Mary’s Hospital) In Sung Moon (The Catholic University of Korea)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.93 No.4

발행연도: 2017.10

수록면: 209 - 216 (8page)

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Purpose: The aim of the present study was to investigate the protective effects of ischemic preconditioning for different periods of time and to elucidate the optimal safe ischemic preconditioning time for renal ischemia-reperfusion (I/R) injury in mice.
Methods: A total of 25 male C57BL/6 mice were randomly divided into 5 groups (sham, I/R, ischemic preconditioning [IP]-3, IP-5, and IP-7 groups), in which the kidney was preconditioned with IP of various durations and then subjected to I/R injury (the last 3 groups). To induce renal ischemia, the left renal pedicle was occluded with a nontraumatic microaneurysm clamp for 30 minutes followed by reperfusion for 24 hours. The effects of IP on renal I/R injury were evaluated in terms of renal function, tubular necrosis, apoptotic cell death and inflammatory cytokines.
Results: Results indicated that BUN and creatinine (Cr) levels increased significantly in the I/R group, but the elevations were significantly lower in IP groups, especially in the IP-5 group. Histological analysis revealed that kidney injury was markedly decreased in the IP-5 group compared with the I/R group, as evidenced by reduced renal necrosis/apoptosis. In addition, IP significantly inhibited gene expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and chemokines (monocyte chemoattractant protein-1). Western blot analysis indicated that the expression levels of Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) were upregulated in the I/R group, while expression was inhibited in the IP groups.
Conclusion: Five-minute IP had the greatest protective effect against I/R injury.

#Ischemic preconditioning #Renal Ischemia-reperfusion injury #TLR4/NF-kB pathway

참고문헌 (22)

참고문헌 신청

[학술지(정기간행물)] - Yoshida T / 2013 / Relaxin protects against renal ischemia-reperfusion injury / Am J Physiol Renal Physiol 305 : F1169 ~ F1176

[학술지(정기간행물)] - Noiri E / 2001 / Oxidative and nitrosative stress in acute renal ischemia / Am J Physiol Renal Physiol 281 : F948 ~ F957

[학술지(정기간행물)] - Chao W. / 2009 / Toll-like receptor signaling: a critical modulator of cell survival and ischemic injury in the heart / Am J Physiol Heart Circ Physiol 296 : H1 ~ H12

[학술지(정기간행물)] - Valeur HS / 2009 / Innate immunity and myocardial adaptation to ischemia / Basic Res Cardiol 104 : 22 ~ 32

[학술지(정기간행물)] - Zhao H / 2014 / Role of Toll-like receptor-4 in renal graft ischemia-reperfusion injury / Am J Physiol Renal Physiol 306 : F801 ~ F811

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