Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with intraperitoneal infection

Zhanwu Li; Wenjun Wang; Xiaozhou Wang; Lei Jiang; Fengyi Wang; Qiang Liu

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자료유형: 학술저널

저자정보: Zhanwu Li (Affiliated Zhongshan Hospital of Dalian University) Wenjun Wang (Affiliated Zhongshan Hospital of Dalian University) Xiaozhou Wang (Affiliated Zhongshan Hospital of Dalian University) Lei Jiang (Affiliated Zhongshan Hospital of Dalian University) Fengyi Wang (Affiliated Zhongshan Hospital of Dalian University) Qiang Liu (Affiliated Zhongshan Hospital of Dalian University)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.93 No.3

발행연도: 2017.9

수록면: 159 - 165 (7page)

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Purpose: To investigate the effects of a sustained-released mixture of vascular endothelial growth factor 165 (VEGF165) and fibrin glue (FG) local administration on postoperative rabbit ileal anastomoses.
Methods: One hundred twenty-eight male and female New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 groups, including 32 animals in each, applied with saline solution, FG, rhVEGF165 and a mixture of rhVEGF165 with FG (VEGF + FG) on the anastomoses, respectively. The incidences of anastomotic leakage were observed. Histopathological examination for inflammatory infiltration, fibroblast proliferation, and capillary vascular proliferation were performed. Then, bursting pressure and hydroxyproline concentrations were assessed in anastomoses sits on postoperative days 3, 5, 7, and 14.
Results: Rabbits in VEGF + FG group had the lowest incidence of leakage (P < 0.05). Histological evaluations revealed that granulation tissue was formed on days 5 after anastomosis; fibroblast proliferation and capillary vascular proliferation were significantly increased on days 7 and 14 in VEGF + FG group. Furthermore, there was a statistically significant difference in the mean bursting pressures between VEGF + FG group and other groups on days 7 and 14 (P < 0.05), and rabbits in VEGF + FG group exhibited a higher concentration than VEGF group (P < 0.05) and FG group (P < 0.05) on day 14.
Conclusion: Administration of VEGF165 mixed with FG to ileal anastomosis accelerates wound healing and enhances the anastomosis by increased angiogenesis.

#Anastomotic leak #Ileum #Vascular endothelial growth factor A #Fibrin tissue adhesive #Delayed-action preparations

참고문헌 (26)

참고문헌 신청

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[학술지(정기간행물)] - Thompson SK / 2006 / Clinical review : healing in gastrointestinal anastomoses, part I / Microsurgery 26 : 131 ~ 136

[학술지(정기간행물)] - Shibuya M. / 2001 / Structure and function of VEGF/VEGF-receptor system involved in angiogenesis / Cell Struct Funct 26 : 25 ~ 35

[학술지(정기간행물)] - Seify H / 2003 / Improvement of TRAM flap viability using human VEGF-induced angiogenesis : a comparative study of delay techniques / Plast Reconstr Surg 112 : 1032 ~ 1039

[학술지(정기간행물)] - Henry TD / 2001 / Intracoronary administration of recombinant human vascular endothelial growth factor to patients with coronary artery disease / Am Heart J 142 : 872 ~ 880

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