지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 160 - 166 (7page)
이용수
초록· 키워드
오류제보하기
To provide a basis for a homogeneous fluorescence resonance energy transfer (FRET) immunoassay for celiac disease, we carried out a FRET experiment using guinea pig tissue transglutaminase (tTG) and antibodies to tTG (anti-tTG) purified from rat serum. Fluorescein was utilized as the probe, and a nonfluorescent dye, QSY 7 served as the quencher. We labeled anti-tTG and tTG with fluorescein isothiocyanate and QSY 7 succinimidyl ester, respectively. Fluoresceinlabeled anti-tTG was the donor, and QSY 7-labeled tTG was the acceptor of the FRET experiment. When we titrated fluorescein-labeled anti-tTG with QSY 7-labeled tTG, we observed a large decrease in the steady-state fluorescence intensity, which was due to strong FRET from fluorescein-labeled anti-tTG to QSY 7-labeled tTG. Using time-resolved fluorescence spectroscopy, we could also observe a decrease in the fluorescence lifetime, which confirms the steady-state data. We expect that these results might be useful in the development of a novel fluorescence immunoassay for an easy screening and follow-up of celiac patients.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - AGA Institute / 2006 / AGA Institute medical position statement on the diagnosis and management of celiac disease / Gastroenterology 131 : 1977 ~ 1980
[학술지(정기간행물)] - Basso D / 2006 / A new indirect chemiluminescent immunoassay to measure anti-tissue transglutaminase antibodies / J Pediatr Gastroenterol 43 : 613 ~ 618
[학술지(정기간행물)] - Basso D / 2009 / Antibodies against synthetic deamidated gliadin peptides for celiac disease diagnosis and follow-up in children / Clin Chem 55 : 150 ~ 157
[학술지(정기간행물)] - Bonamico M / 2008 / Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor celiac disease follow-up / Aliment Pharmacol Ther 28 : 364 ~ 370
[학술지(정기간행물)] - Bonamico M / 2001 / Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease / Am J Gastroenterol 96 : 1536 ~ 1540
[학술지(정기간행물)] - Basso D / 2006 / A new indirect chemiluminescent immunoassay to measure anti-tissue transglutaminase antibodies / J Pediatr Gastroenterol 43 : 613 ~ 618
[학술지(정기간행물)] - Basso D / 2009 / Antibodies against synthetic deamidated gliadin peptides for celiac disease diagnosis and follow-up in children / Clin Chem 55 : 150 ~ 157
[학술지(정기간행물)] - Bonamico M / 2008 / Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor celiac disease follow-up / Aliment Pharmacol Ther 28 : 364 ~ 370
[학술지(정기간행물)] - Bonamico M / 2001 / Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease / Am J Gastroenterol 96 : 1536 ~ 1540
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UCI(KEPA) : I410-ECN-0101-2017-510-002026362