지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 284 - 290 (7page)
이용수
초록· 키워드
오류제보하기
In this research, recombinant human cytotoxic Tlymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced by transgenic rice cells. RAmy3D promoter was used for overcome the limitation of low expression level in transgenic plant cells, and the secretion of target protein was accomplished by signal peptide. However, the RAmy3D promoter system which can be induced only by sugar starvation causes the decrease of cell viability. As a result, cell death promotes the release of protease which degrades the target proteins. The protein stability and productivity can be significantly influenced by proteolysis activity. Therefore, development of new strategies are necessary for the in situ recovery of target proteins from cell culture media. In this study, in situ recovery was performed by various strategies. Direct addition of Protein A resin with nylon bag leads to cell death by increased shear stress and decrease in production of hCTLA4Ig by protease. Medium exchange through modified flask could recover hCTLA4Ig with high cell viability and low protease activity, on the other hand, the productivity was lower than that of control. When in situ recovery was conducted at day 7 after induction in air-lift bioreactor, 1.94-fold of hCTLA4Ig could be recovered compared to control culture without in situ recovery. Consequently, in situ recovery of hCTLA4Ig from transgenic rice cell culture could enhance productivity significantly and prevent degradation of target proteins effectively.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (16)
참고문헌 신청
[학술지(정기간행물)] - Lee, S. -J. / 2007 / Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures / Protein Expres. Purif 51 : 293 ~ 302
[학술지(정기간행물)] - W. R. Strohl / 2015 / Fusion proteins for half-life extension of biologics as a strategy to make biobetters / Biodrugs 29 : 215 ~ 239
[학술지(정기간행물)] - Toyofuku, K. / 1998 / Promoter elements required for sugar-repression of the RAmy3D gene for α-amylase in rice / FEBS Lett 428 : 275 ~ 280
[학술지(정기간행물)] - Takatsuka, C. / 2004 / 3-Methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions / Plant Cell Physiol 45 : 265 ~ 274
[학술지(정기간행물)] - Kwon, J.-Y. / 2012 / Application of anoxia with glucose addition for the enhanced production of hCTLA4Ig in transgenic rice suspension cell cultures / Enzyme Microb. Tech 50 : 298 ~ 303
[학술지(정기간행물)] - W. R. Strohl / 2015 / Fusion proteins for half-life extension of biologics as a strategy to make biobetters / Biodrugs 29 : 215 ~ 239
[학술지(정기간행물)] - Toyofuku, K. / 1998 / Promoter elements required for sugar-repression of the RAmy3D gene for α-amylase in rice / FEBS Lett 428 : 275 ~ 280
[학술지(정기간행물)] - Takatsuka, C. / 2004 / 3-Methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions / Plant Cell Physiol 45 : 265 ~ 274
[학술지(정기간행물)] - Kwon, J.-Y. / 2012 / Application of anoxia with glucose addition for the enhanced production of hCTLA4Ig in transgenic rice suspension cell cultures / Enzyme Microb. Tech 50 : 298 ~ 303
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UCI(KEPA) : I410-ECN-0101-2017-470-001981647