지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 277 - 283 (7page)
이용수
초록· 키워드
오류제보하기
In this system, rice cells were genetically modified to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter induced by sugar depletion. Even though the target protein fused with signal sequence peptide, plant cell wall can be a barrier against secretion of recombinant proteins. Therefore, hCTLA4Ig can be trapped inside cell wall or remained in intracellular space. In this study, to enhance the secretion of hCTLA4Ig from cytoplasm and cell walls into the medium, permeabilizing agents, such as dimethyl sulfoxide (DMSO), Triton X-100 and Tween 20, were applied in transgenic rice cell cultures. When 0.5% (v/v) of DMSO was added in sugar-free medium, intracellullar hCTLA4Ig was increased, on the other hand, the secreted extracellular hCTLA4Ig was lower than that of control. DMSO did not give permeable effects on transgenic rice cell cultures. And Triton X-100 was toxic to rice cells and also did not give enhancing permeability of cells. When 0.05% (v/v) Tween 20 was added in rice cell cultures, however, intracellular hCTLA-4Ig was lower than that of control cultures. And the maximum 44.76 mg/L hCTLA4Ig was produced for 10 days after induction, which was 1.4-fold increase compared to that of control cultures. Especially, Tween 20 at 0.05% (v/v) showed the positive effect on the secretion of hCTLA4Ig though the decrease of intracellular hCTLA4Ig. Also, Tween 20 as a non-toxic surfactant did not affect the cell growth, cell viability and protease activity. In conclusion, secretion of hCTLA4Ig could be increased by enhancing permeability of cells regardless of the cell growth, cell viability and protease activity.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (21)
참고문헌 신청
Grabowski, G. A. / 2014 / Taliglucerase alfa: An enzyme replacement therapy using plant cell expression technology / Mol. Gen. Metabol 112:1~8
Davidson, E. / 2015 / Mechanism of binding to ebola virus glycoprotein by the ZMapp, ZMAb, and MB-003 cocktail antibodies / J. Virol 89:10982~10992
Tekoah, Y. / 2015 / Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience / Plant Biotechnol. J 13:1199~1208
Shin, Y. -J. / 2010 / N-linked glycan analysis of glycoproteins secreted from rice cell suspension cultures under sugar starvation / Enzyme Microb. Tech 47:189~193
Zhang, N / 2016 / High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: Process development / Biotechnol. J 11:497~506
Davidson, E. / 2015 / Mechanism of binding to ebola virus glycoprotein by the ZMapp, ZMAb, and MB-003 cocktail antibodies / J. Virol 89:10982~10992
Tekoah, Y. / 2015 / Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience / Plant Biotechnol. J 13:1199~1208
Shin, Y. -J. / 2010 / N-linked glycan analysis of glycoproteins secreted from rice cell suspension cultures under sugar starvation / Enzyme Microb. Tech 47:189~193
Zhang, N / 2016 / High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: Process development / Biotechnol. J 11:497~506
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
형질전환 벼 현탁세포 배양에서 hCTLA4Ig의 in situ 회수
KSBB Journal
2016 .12
-
Comparison of Nitrogen Sources for Production of hCTLA4Ig in Transgenic Rice Cell Cultures
한국생물공학회 학술대회
2016 .04
-
Effects of Amino Acids on Cell Viability and hCTLA4Ig Production in Transgenic Rice Cell Cultures
한국생물공학회 학술대회
2015 .04
-
형질전환 벼 현탁세포 배양에서 혼합효율과 조정배지가 hCTLA4Ig 생산에 미치는 영향
KSBB Journal
2015 .12
-
Effects of Inoculum Age and Density on hCTLA4Ig Production in Transgenic Rice Cell Cultures
한국생물공학회 학술대회
2015 .04
-
α2,6-Sialyltransferase 과발현을 통한 인간형 시알산 부가 hCTLA4-Ig 생산 CHO 세포주 제작
KSBB Journal
2017 .09
-
Reduction of Protein Degradation by Using Protein-stabilizing Agents in Transgenic Rice Cell Cultures
한국생물공학회 학술대회
2015 .10
-
Enhancing hCTLA4-Ig productivity by controlling the feeding time of 4-phenylbutyrate in CHO cell cultures
한국생물공학회 학술대회
2017 .04
-
Increased hCTLA4-Ig production by regulating feeding time of 4-phenylbutyrate in CHO cell cultures
한국생물공학회 학술대회
2017 .10
-
Effects of 4-phenylbutyrate addition time on the productivity and glycan structure of hCTLA4-Ig in CHO cell culture
한국생물공학회 학술대회
2018 .10
-
Effects of addition time of 4-phenylbutyrate on the productivity and glycan structure of hCTLA4-Ig in CHO cell culture
한국생물공학회 학술대회
2018 .04
-
The Yin and Yang of RNA surveillance in B lymphocytes and antibody-secreting plasma cells
BMB Reports
2019 .01
-
Substitution of Glutamine by Glutamate and Alanyl-glutamine to Reduce Ammonia Production in Transgenic Rice Cell Cultures
한국생물공학회 학술대회
2015 .10
-
소듐, 리튬금속화재에 대한 불활성 기체 소화약제의 적용성 분석
한국위험물학회지
2020 .12
-
IGS 뉴스 (News letter, Volume 31, Issue 1, 2015)
한국지반신소재학회지
2015 .07
-
Accuracy Evaluation of IGS-RTS Corrections to Stand-Alone Positioning Based on GPS Code-Pseudorange Measurements
Journal of Positioning, Navigation, and Timing
2016 .01
-
남극 기준점 측지측량 및 IGS등록 방안에 관한 연구
대한토목학회 학술대회
2023 .10
-
Silver-PDT Effect on Co-Evaporated CIGS IGS(Cu(In,Ga)Se₂) Thin Film Solar Cells
AFORE
2019 .11
-
Study on Cell Culture Process by using Single-use Cell Culture System
한국생물공학회 학술대회
2017 .04
-
Alloy 600의 결정립계 산화에 대한 표면 변형의 영향
Corrosion Science and Technology
2020 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
유전자 형질전환을 통한 쌀 전분 분해효소 재조합 효모균주의 개발과 발효특성조사
이자연
,
진종언
,
배석
미생물학회지
2016
.06
MdMADS2 유전자가 도입된 사과 형질전환체의 개화유도 유전자 발현 분석
천재안
,
조강희
,
김세희
외 3명
한국원예학회 학술발표요지
2016
.10
암 치료를 위한 항체치료제에 대한 고찰: 면역항암제
유한진
,
홍세영
,
권미지
외 3명
KSBB Journal
2020
.06
형질전환 벼 현탁세포 배양에서 세포 사멸 억제를 통한 hCTLA4Ig 생산성 증대
김명식
,
남형진
,
김민섭
외 2명
KSBB Journal
2013
.08
형질전환 벼 세포 배양에서 Sorbitol과 Hexokinase 저해제 조합 전략을 통한 Acid α-glucosidase 생산성 증대
정창현
,
최홍열
,
복다은
외 5명
KSBB Journal
2021
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-470-001981632