Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand

Attakorn Palasuwan; Duangdao Palasuwan; Aongart Mahittikorn; Rachatawan Chiabchalard; Valery Combes; Supaluk Popruk

추천

검색

질문

자료유형: 학술저널

저자정보: Attakorn Palasuwan (Chulalongkorn University) Duangdao Palasuwan (Chulalongkorn University) Aongart Mahittikorn (Mahidol University) Rachatawan Chiabchalard (Mahidol University) Valery Combes (University of Technology) Supaluk Popruk (Mahidol University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.4

발행연도: 2016.8

수록면: 455 - 460 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa–Kishino–Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524–KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages.

#Blastocystis #subtype #zoonotic risk #Chao Phraya River #Thailand

참고문헌 (30)

참고문헌 신청

[학술지(정기간행물)] - Tan KS / 2008 / New insights on classification, identification, and clinical relevance of Blastocystis spp / Clin Microbiol Rev 21 : 639 ~ 665

[학술지(정기간행물)] - Wong KH / 2008 / Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore / Parasitol Res 102 : 663 ~ 670

[학술지(정기간행물)] - Roberts T / 2011 / Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples / Am J Trop Med Hyg 84 : 308 ~ 312

[학술지(정기간행물)] - Alfellani MA / 2013 / Genetic diversity of Blastocystis in livestock and zoo animals / Protist 164 : 497 ~ 509

[학술지(정기간행물)] - Rene BA / 2009 / Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients / Am J Trop Med Hyg 80 : 588 ~ 592

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!