Hemozoin Pigment: An Important Tool for Low Parasitemic Malarial Diagnosis

Sarita Mohapatra; Arnab Ghosh; Ruchi Singh; Dhirendra Pratap Singh; Bhawna Sharma; Jyotish Chandra Samantaray; Manorama Deb; Rajni Gaind

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자료유형: 학술저널

저자정보: Sarita Mohapatra (All India Institute of Medical Sciences) Arnab Ghosh (All India Institute of Medical Sciences) Ruchi Singh (Safdarjung Hospital) Dhirendra Pratap Singh (Safdarjung Hospital) Bhawna Sharma (Vardhaman Mahavir Medical College and Safdarjung Hospital) Jyotish Chandra Samantaray (All India Institute of Medical Sciences) Manorama Deb (Vardhaman Mahavir Medical College and Safdarjung Hospital) Rajni Gaind (Vardhaman Mahavir Medical College and Safdarjung Hospital)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.4

발행연도: 2016.8

수록면: 393 - 397 (5page)

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Low parasitemic condition in malaria remains a diagnostic challenge; as the available diagnostic methods failed to detect. Currently, hemozoin (Hz) pigment is gaining attention in the diagnosis of malaria. The major drawback is ease of detection of Hz in routine practice. A pilot study was conducted to evaluate the role of Hz pigment and to compare the performance of quantitative buffy coat assay (QBC) and PCR in such conditions. Clinically suspected cases of malaria were examined by both Giemsa stain and immunochromatographic test (ICT). Samples positive by ICT and negative by Giemsa stain were further examined by nested PCR targeting 18S rRNA and QBC for the presence of malaria parasites and pigments. Thirty blood samples fulfilled the inclusion criteria out of which 23 were Plasmodium vivax (Pv), 4 Plasmodium falciparum (Pf), and 3 mixed (Pv and Pf) by immunochromatographic test. Twenty-one out of 30 (70%) were positive by nested PCR in comparison to 25/30 (83%) by QBC. Samples containing both malaria parasites and Hz pigment by QBC completely showed concordance with the PCR result. However, 61% of total samples containing only Hz pigment were observed positive by PCR. Hz pigment remains an important tool for malaria diagnosis. Identification of leukocytes containing pigments by QBC not only indicates recent malarial infections but also puts light on severity of the disease.
QBC assay is a rapid, highly sensitive, and cost-effective method to detect malaria parasites and Hz pigment especially in low parasitemic conditions.

#Plasmodium vivax #Plasmodium falciparum #low parasitemia #hemozoin pigment #QBC #nested PCR

참고문헌 (12)

참고문헌 신청

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