An Overview of Different Techniques on the Microbial Community Structure, and Functional Diversity of Plant Growth Promoting Bacteria

Kiyoon Kim; Rashedul Islam; Abitha Benson; Manoharan Melvin Joe; Walitang Denver; Mak Chanratan; Poulami Chatterjee; Yeongyeong Kang; Tongmin Sa

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자료유형: 학술저널

저자정보: Kiyoon Kim (Chungbuk National University) Rashedul Islam (Chungbuk National University) Abitha Benson (Chungbuk National University) Manoharan Melvin Joe (Chungbuk National University) Walitang Denver (Chungbuk National University) Mak Chanratan (Chungbuk National University) Poulami Chatterjee (Chungbuk National University) Yeongyeong Kang (Chungbuk National University) Tongmin Sa (Chungbuk National University)

저널정보: 한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제49권 제2호

발행연도: 2016.4

수록면: 144 - 156 (13page)

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Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

#PLFA #FAME analysis #CLPP #SIR #DGGE #TGGE #T-RFLP #SSCP #RFLP #ARDRA #RISA

참고문헌 (93)

참고문헌 신청

Allison, S.D. and J.B.H. Martiny. 2008. Resistance, resilience, and redundancy in microbial communities. Proc. Nat. Acad. Sci. 105:1151-1159.

Amann, R., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.

Ananyeva, N.D., E.A. Susyan, and E.G. Gavrilenko. 2011. Determination of the soil microbial biomass carbon using the method of substrate-induced respiration. Eur. Soil Sci. 44(11):1215-1221.

Banning, N.C., B.M. Lalor, W.R. Cookson, A.H. Grigg, and D.V. Murphy. 2012. Analysis of soil microbial community level physiological profiles in native and post-mining rehabilitation forest: Which substrates discriminate?. Appl. Soil Ecol. 56:27-34.

Ben-David, E.A., E. Zaady, Y. Sher, and A. Nejidat. 2011. Assessment of the spatial distribution of soil microbial communities in patchy arid and semi-arid landscapes of the Negev Desert using combined PLFA and DGGE analyses. FEMS. Microbiol. Ecol. 76(3):492-503.

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