Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products

Giimaa Narantsogt; Arim Min; Young Hee Nam; Young Ah Lee; Kyeong Ah Kim; Gurbadam Agvaandaram; Temuulen Dorjsuren; Jamel El-Benna; Myeong Heon Shin

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저자정보: Giimaa Narantsogt (Yonsei University College of Medicine) Arim Min (Yonsei University College of Medicine) Young Hee Nam (Yonsei University College of Medicine) Young Ah Lee (Yonsei University College of Medicine) Kyeong Ah Kim (Yonsei University College of Medicine) Gurbadam Agvaandaram (Mongolian National University of Medical Sciences) Temuulen Dorjsuren (Mongolian National University of Medical Sciences) Jamel El-Benna (Universite Paris & Denis Diderot) Myeong Heon Shin (Yonsei University College of Medicine)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.5

발행연도: 2015.10

수록면: 597 - 603 (7page)

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Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47phox in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.

#Trichomonas vaginalis #secretory product #mast cell #MAPK #ROS generation #exocytotic degranulation

참고문헌 (34)

참고문헌 신청

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[학술지(정기간행물)] - Brill JR / 2010 / Diagnosis and treatment of urethritis in men / Am Fam Physician 81 : 873 ~ 878

[학술지(정기간행물)] - Nam YH / 2011 / Leukotriene B4 receptor BLT-mediated phosphorylation of NF-κB and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products / Microbes Infect 13 : 1211 ~ 1220

[학술지(정기간행물)] - Seo MY / 2014 / Inflammatory response of prostate epithelial cells to stimulation by Trichomonas vaginalis / Prostate 74 : 441 ~ 449

[학술지(정기간행물)] - Johnston VJ / 2008 / Global epidemiology and control of Trichomonas vaginalis / Curr Opin Infect Dis 21 : 56 ~ 64

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