지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 442 - 450 (9page)
이용수
초록· 키워드
오류제보하기
This study was conducted to investigate the effect of Psy-2A-CrtI (PAC), a genetically modified (GM) rice with enhanced β-carotene, on the soil microbial community. The soil used to cultivate GM rice and its wild-type, Nakdong, was analyzed for population density, denaturing gradient gel electrophoresis (DGGE), and pyrosequencing. It was found that the bacterial, fungal and actinomycetes population densities of the PAC soils were within the range of those of the non-GM rice cultivar, Nakdong. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The pyrosequencing result showed a temporal difference in microorganism taxon and distribution ratio, but no significant difference between GM and non-GM was found. The persistence of the transgene DNA in the plant and surrounding soil were investigated for different time periods. There were differences in the persistence within the plant depending on the gene, but they could not be detected after 5 weeks. Also the transgenes were not detected in the surrounding soil. These results indicate that soil microbial communities are unaffected by the cultivation of a PAC rice within the experimental time frame.
목차
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - AHN, JAE-HYUNG / 2006 / Improvement of PCR Amplification Bias for Community Structure Analysis of Soil Bacteria by Denaturing Gradient Gel Electrophoresis / Journal of Microbiology and Biotechnology 16 (10) : 1561 ~ 1569
[학술지(정기간행물)] - Bardgett R. D. / 1999 / Below-ground herbivory promotes soil nutrient transfer and root growth in grassland / Ecol. Lett 2 : 357 ~ 360
[학술지(정기간행물)] - Barriuso J. / 2012 / Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period / PLoS ONE 7 : e35481 ~
[학술지(정기간행물)] - Bertolla S. / 1999 / Horizontal gene transfer in environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms / Res. Microbiol 150 : 375 ~ 384
[학술지(정기간행물)] - Cruz-Martínez K. / 2012 / Effect of rainfall-induced soil geochemistry dynamics on grassland soil microbial communities / Appl. Environ. Microbiol 78 : 7587 ~ 7595
[학술지(정기간행물)] - Bardgett R. D. / 1999 / Below-ground herbivory promotes soil nutrient transfer and root growth in grassland / Ecol. Lett 2 : 357 ~ 360
[학술지(정기간행물)] - Barriuso J. / 2012 / Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a four-year cultivation period / PLoS ONE 7 : e35481 ~
[학술지(정기간행물)] - Bertolla S. / 1999 / Horizontal gene transfer in environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms / Res. Microbiol 150 : 375 ~ 384
[학술지(정기간행물)] - Cruz-Martínez K. / 2012 / Effect of rainfall-induced soil geochemistry dynamics on grassland soil microbial communities / Appl. Environ. Microbiol 78 : 7587 ~ 7595
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UCI(KEPA) : I410-ECN-0101-2016-521-002012994