지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 1,508 - 1,521 (14page)
이용수
초록· 키워드
오류제보하기
Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form β-barrel with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of 4.2±0.7 and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.
목차
Abstract
Ⅰ. Introduction
Ⅱ. Materials and methods
Ⅲ. Results and Discussion
References
참고문헌 (31)
참고문헌 신청
[학술지(정기간행물)] - Altschul S. F. / 1997 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
[학술지(정기간행물)] - Alves N. Jr / 2010 / Diversity and pathogenic potential of vibrios isolated from Abrolhos Bank coral / Environ Microbiol Rep 2 (1) : 90 ~ 95
[학술지(정기간행물)] - Austin B. / 2006 / Vibrio harveyi: a significant pathogen of marine vertebrates and in vertebrates / Lett Appl Microbiol 43 : 119 ~ 124
[단행본] - Edward J. N. / 1996 / Fish diseases: Diagnosis and treatment / Iowa state university Press
[학술지(정기간행물)] - Ellis T. N. / 2010 / Virulence and immunomodulatory roles of bacterial outer membrane vesicles / Microbiol Mol Biol Rev 74 : 81 ~ 94
[학술지(정기간행물)] - Alves N. Jr / 2010 / Diversity and pathogenic potential of vibrios isolated from Abrolhos Bank coral / Environ Microbiol Rep 2 (1) : 90 ~ 95
[학술지(정기간행물)] - Austin B. / 2006 / Vibrio harveyi: a significant pathogen of marine vertebrates and in vertebrates / Lett Appl Microbiol 43 : 119 ~ 124
[단행본] - Edward J. N. / 1996 / Fish diseases: Diagnosis and treatment / Iowa state university Press
[학술지(정기간행물)] - Ellis T. N. / 2010 / Virulence and immunomodulatory roles of bacterial outer membrane vesicles / Microbiol Mol Biol Rev 74 : 81 ~ 94
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
EFFECT OF Bacillus spp. TO CONTROL PATHOGENIC BACTERIA Vibrio harveyi IN PACIFIC WHITE SHRIMP CULTURE
한국수산과학회 양식분과 학술대회
2015 .05
-
모노퍼설페이트 화합물의 Vibrio harveyi에 대한 살균력 및횐다리새우(Litopenaeus vannamei)에서의 독성
한국수산과학회지
2015 .10
-
육상 사육 황복(Takifugu obscurus)친어의 Vibrio harveyi감염증
수산해양교육연구
2021 .06
-
USE OF FTA CARDS FOR QUANTITATIVE PCR DETECTION OF Vibrio harveyi AND Vibrio campbelli TOXIN GENES AND GENE EXPRESSION WITHIN SHRIMP POND WATERSAMPLES
한국수산과학회 양식분과 학술대회
2015 .05
-
Identification of Vibrio species isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju Island, South Korea
Fisheries and Aquatic Sciences
2019 .01
-
IDENTIFICATION OF Photobacterium damselae, Vibrio harveyi, Vibrio ichthyoenteri AND Vibrio scophthalmi ISOLATED IN KOREA BY MULTIPLEX PCR
한국수산과학회 양식분과 학술대회
2015 .05
-
EFFECT OF BIOFLOCS ON GROWTH PERFORMANCE, FEED UTILIZATION AND INNATE IMMUNITY OF WHITE SHRIMP Litopenaeus vannamei AGAINST Vibrio harveyi
한국수산과학회 양식분과 학술대회
2015 .05
-
Calculating the length of olive flounder (Paralichthys olivaceus) using image processing
한국수산과학회 양식분과 학술대회
2022 .10
-
EFFECT OF Macleaya cordata EXTRACT ON PREVENTION OF Vibrio harveyi INFECTION IN PACIFIC WHITE SHRIMP Litopenaeus vannamei
한국수산과학회 양식분과 학술대회
2015 .05
-
Processing and quality properties of olive flounder Paralichthys olivaceus balls product
한국수산과학회 양식분과 학술대회
2015 .10
-
제주지역 양식 넙치(Paralichthys olivaceus)에서 분리한 어병세균 내 Erythromycin 내성 유전자 분석
한국수산과학회지
2018 .08
-
EFFECTS OF PROBIOTICS ON GROWTH, SURVIVAL, TOTAL Vibrio spp. IN INTESTINE AND SURVIVAL RATE AFTER CHALLENGE WITH V. harveyi OF PACIFIC WHITE SHRIMP Litopenaeus vannamei REARING
한국수산과학회 양식분과 학술대회
2015 .05
-
Optimum dietary protein level for growth performances and blood parameters of family selected olive flounder, Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2017 .05
-
CO-INFECTION OF INFECTIOUS MYONECROSIS VIRUS (IMNV) AND Vibrio harveyi IN PACIFIC WHITE SHRIMP Litopenaeus vannamei
한국수산과학회 양식분과 학술대회
2015 .05
-
Effect of fermented tuna by-product containing diets with and without Song-gang stone® on growth, immune response and disease resistance in juvenile olive flounder Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2017 .05
-
Genomic analysis of fish pathogen Vibrio scophthalmi isolated from Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2022 .10
-
Purification and Determination of Two Novel Antioxidant Peptides from Flounder Fish (Paralichthys olivaceus)
한국식품영양과학회 학술대회발표집
2016 .10
-
DEVELOPMENT OF A GOLDEN OLIVE FLOUNDER (Paralichthys olivaceus) STRAIN THROUGH QUANTITATIVE AND MOLECULAR APPROACHES
한국수산과학회 양식분과 학술대회
2015 .05
-
Molecular Cloning and Characterization of melanin synthesis related genes in Olive flounder, Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2015 .10
-
RNA-seq data of olive flounder (Paralichthys olivaceus) according to water temperature
한국수산과학회 양식분과 학술대회
2019 .05
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0