Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene

Ju-Hua Wang; Xiu-Heng Xue; Jie Zhou; Cai-Yun Fan; Qian-Qian Xie; Pan Wang

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자료유형: 학술저널

저자정보: Ju-Hua Wang (Anhui Agriculture University) Xiu-Heng Xue (Anhui Agriculture University) Jie Zhou (Anhui Agriculture University) Cai-Yun Fan (Anhui Agriculture University) Qian-Qian Xie (Anhui Agriculture University) Pan Wang (Anhui Agriculture University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.3

발행연도: 2015.6

수록면: 335 - 339 (5page)

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Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca2+, Mg2+, K+, and HCO₃- in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

#Cryptosporidium andersoni #cloning #iron transportation #ATP-binding cassette transporter protein

참고문헌 (17)

참고문헌 신청

[학술지(정기간행물)] - Xiang Y / 2010 / Molecular identification of Cryptosporidium ryanae isolate from dairy cows in Harbin / Chinese J Zoon 26 : 144 ~ 146

[학술지(정기간행물)] - Zhu M / 2003 / An animal model for Cryptosporidium parvum in mice / Chinese J Parasit Dis Control 16 : 13 ~ 15

[학술지(정기간행물)] - Hijjawi NS / 2002 / Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium / Int J Parasitol 32 : 1719 ~ 1726

[학술지(정기간행물)] - Wen PC / 2011 / Conformational coupling of the nucleotide-binding and the transmembrane domains in ABC transporters / Biophys J 101 : 680 ~ 690

[학술지(정기간행물)] - LeChevallier MW / 2003 / Comparison of method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters / Appl Environ Microbiol 69 : 971 ~ 979

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