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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제26권 제1호
발행연도
1996.6
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1 - 8 (8page)

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Human cytomegalovirus (HCMV) replication and Ca" response in human cell lines of neuronal origin were investigated. SK-N-SH (neuroblastoma cells) and A172 cells (glioblastoma cells) were used. SK-N-SH cells were permissive for HCMV multiplication with a delay of one day compared to virus multiplication in human embryo lung (HEL) cells. The delay of HCMV multiplication in SK-N-SH cells appeared to be correlated with a delay in the Ca" response. The cytoplasmic free Ca" concentration ([Ca"];) began to increase at 12 h p.I. In HCMV-infected SK- N-SH cells, while [Ca"]; increase in HCMV-infected HEL cells was observed as early as 3 h p.I. On the whole, the level of the increase in [Ca"]; in SK-N-SH cells was about 30% of that in HEL cells. On the other hand, in A172 cells infected with HCMV, neither production of infectious virus nor detectable increase in [Ca"]; was observed. Treatment with TPA of HCMV-in- fected SK-N-SH cells resulted in [Ca"]; increase at 6 h. p.I. The stimulatory effect of TPA on HCMV- induced [Ca"]; increase continued until 12 h p.I., but TPA failed to stimulate the Ca' response in SK-N-SH cells at 24 h p.I., suggesting that the effect of TPA had disappeared in SK- N-SH cells at that time point. In conclusion, SK-N-SH cells are permissive for HCMV replication and the delay in Ca" response may be a consequence of the lower responsiveness of SK-N-SH cells than HEL cells to HCMV infection.

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