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자료유형
학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제22권 제1호
발행연도
1992.6
수록면
27 - 36 (10page)

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This experiment was carried out to investigate the immunogenicity in swine of the inactivated Aujeszkys disease virus(NYJ-1 strain of Korean isolates) antigens per se(Group 1) or supplemented with oil-adjuvant (Group 2). The infectivity of the virus was completely inactivated by treatment with 0.001M binary ethylenimine (BEI) for 18 hours, or treatment with 0.1% formalin for 24 hours. In clinical observation, none of the experimental swine showed a specific clinical sign following inoculation of the antigens. But after challenge with the virulent ADV at 10TCID per 0.2ml, fever, nervous sings and diarrhea were manifested in 2 out of 4 heads in Group 1 (no adjuvant) and 2 out of 3 heads in Group 3 (control), but no specific signs were observed in Group 2 (oiladjuvant). The weight gains were rneasured for 1 week after booster and 1 week after challenge in- oculations respectively. After booster there were little differences among three groups, but after challenge the weight gains of Group 1 and 3 were lower than that of Group 2. The virus was excreted from the nasal cavity of all three groups on the 2nd to 6th days after challenge. The excretion rate of Group 2 was lower than those of Group 1 and 3. Antibody responses were measured by virus neutralization (VN), agar gel precipitation and immunoenzyme assays. After booster inoculation, Group 1 (no adjuvant) revealed 4.2 to 4.6 and Group 2 (oil-adjuvant), 56 to 117 of VN titers. After challenge with the virulent ADV, Group 1 showed 24-56, Group 2, 482-521 and Group 3, 4-8.3 of VN titers, respectivley. In detection of ADV specific antibody in nasal excretions, all showed negative in both of agar gel precipitation test and VN test, but partial or positive reactions were observed in immunoenzyme assays. Using leukocyte adherence inhibition and skin tests, more enhancing cellular immune responses were shown in Group 2 compared with Group 1 and 3.

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