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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제21권 제2호
발행연도
1991.12
수록면
163 - 172 (10page)

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Aujeszkys disease virus (ADV), NYJ-1 strain, isolated from the diseased piglets in Korea was inactivated with binary ethyleneimine or formalin and absorbed in aluminum hydroxide gel. Safety and humoral and cellular immunogenicity of the antigens were investigated in mouse, guinea pig and piglets. In mouse inoculation test, the antigens showed 100% of safety and induced a significant level of ADV antibody. The protective potency following intracerebral challenge with the virulent ADV at 10TCID/0.2 ml were 60% to 64.3%. In guinea pig inoculation test, it was found that the antigens were quite safe, inducing 63.2 to 124.9 of virus neutralization (VN) titers at the 5th week post inoculation (pi). The protectivity of the antigens against the challenge of the virulent ADV were 62. 5% to 75.0%. In piglet inoculation test, the antigens were quite safe after the primary and booster injections, and induced 28.7 to 39.1 of VN titers at the 5th week pi. The high levels of ADV antibodies were also recognized by agar gel precipitation test, latex agglutination test and immunoenzyme assays. Leucocyte adherence inhibition(LAI) test for the piglets revealed 22.3+3.8% to 32.8+6.9% of LAI index at the 4th week pi, and the evident reactions of delayed type hypersensitivity was observed in subcutaneous inoculation of the antigen to the imrnunized piglets. Following the challenge, the immunized piglets showed none of specific clinical signs of AD and revealed the higher gain of body weight, and the lower secretion of ADV from the nasal cavity as compared with the control group.

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