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자료유형
학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제18권 제1호
발행연도
1988.6
수록면
21 - 33 (13page)

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The sera from the risk group of acquired immunodeficiency syndrorne (AIDS) in Korea were tested for anti-HIV(human immunodeficiency virus) antibody utilizing several serologic techniques. The purposes of this study were to investigate the presence of HIV in our country through demonstration of anti-HIV antibody and to evaluate the techniques used in this study and also to establish the antibody test system which is reliable as well as simple. All sera to be tested were heat treated(56C, 30 min.) and diluted in 1: 32 with phosphate buffered saline(PBS; 0.01 M, pH 7.2). Primarilly, sera were tested by indirect immunofluorescent antibody(IFA) technique and some sera were further tested by western blot or fluorescence reduction neutralization (FRN) tests if the score can not be made by preliminary IFA test. 40 male homosexuals, 560 prostitutes, 23 hemophiliacs and 852 venereal disease patients were tested. None of these showed positive for anti-HIV antibody. This result indicates that the prevalence of HIV in Korea is still very low at present time. The methods used in this study could be established for detection of anti-HIV antibody. Espiccially, IFA test using HIV infected TALL-1 cells was enough sensitive, specific and simple as well. Then western blot and FRN tests were coincided with IFA in the results. Thus the confirmation system by western blot and FRN tests followed by IFA screening could be employed for seroepidemiological study of HIV.

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