지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.4
- 수록면
- 82 - 90 (9page)
이용수
초록· 키워드
오류제보하기
Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGMCSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was
7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.
7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (11)
참고문헌 신청
1. [학술지(정기간행물)] - Aoyagi H. / 1998 / Alginate promotes production of various enzymes by Catharanthus roseus cells / Plant Cell Rep 17 : 243 ~ 247
2. [학술지(정기간행물)] - Bodeutsch, T / 2001 / The effect of immobilization on recombinant protein production in plant cell culture / Plant Cell Rep 20 : 562 ~ 566
3. [학술지(정기간행물)] - Durocher, Y. / 2009 / Expression systems for therapeutic glycoprotein production / Curr. Opin. Biotechnol 6 : 700 ~ 707
4. [단행본] - Glick, B. R. / 1998 / Molecular Biotechnology:Principles and Applications of Recombinant DNA / American Society for Microbiology Press
5. [학술지(정기간행물)] - James, E. A. / 2001 / The production of foreign protein from genetically modified plant cells / Adv. Biochem. Eng. Biotechnol 72 : 127 ~ 156
2. [학술지(정기간행물)] - Bodeutsch, T / 2001 / The effect of immobilization on recombinant protein production in plant cell culture / Plant Cell Rep 20 : 562 ~ 566
3. [학술지(정기간행물)] - Durocher, Y. / 2009 / Expression systems for therapeutic glycoprotein production / Curr. Opin. Biotechnol 6 : 700 ~ 707
4. [단행본] - Glick, B. R. / 1998 / Molecular Biotechnology:Principles and Applications of Recombinant DNA / American Society for Microbiology Press
5. [학술지(정기간행물)] - James, E. A. / 2001 / The production of foreign protein from genetically modified plant cells / Adv. Biochem. Eng. Biotechnol 72 : 127 ~ 156
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