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논문 기본 정보

자료유형
학술저널
저자정보
오영훈 (한국화학연구원) 강경희 (한국화학연구원) 신지훈 (한국화학연구원) 송봉근 (한국화학연구원) 이승환 (전남대학교) 이상엽 (한국과학기술원) 박시재 (명지대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal 제29권 제6호
발행연도
2014.12
수록면
443 - 447 (5page)

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Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 (PhaC1<SUB>Ps6-19</SUB>) and engineered C. propionicum propionyl-CoA transferase (Pct<SUB>Cp</SUB>), respectively, were expressed in E. coli W to construct key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli W expressing the phaC1437 gene and the pct540 gene could synthesize P(3HB-co-13mol%LA) up to the polymer content of 31.3 wt% when it was cultured in chemically defined MR medium containing 20 g/L of sucrose and 2 g/L of sodium 3-hydroxybutyrate. When Ralstonia eutropha phaAB genes were additionally expressed to provide 3-hydroxybutyrate-CoA (3HB-CoA) from sucrose, P(3HB-co-16mol%LA) could be synthesized from sucrose as a sole carbon source without supplement of sodium 3-hydroxybutyrate in culture medium, but the PHA content was decreased to 12.2 wt%. The molecular weight of P(3HB-co-16 mol%LA) synthesized in E. coli W using sucrose as carbon source were 1.53×10<SUP>4</SUP> (M<SUB>n</SUB>) and 2.78×10<SUP>4</SUP> (M<SUB>w</SUB>), respectively, which are not different from those that have previously been reported by other recombinant E. coli strains. Engineered E. coli strains developed in this study should be useful for the production of lactate-containing PHAs from sucrose, one of the most abundant and least expensive carbon sources.

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Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2016-470-000965003