지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.8
- 수록면
- 244 - 249 (6page)
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초록· 키워드
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Biosynthesis pathway of medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acid β-oxidation pathway was constructed in recombinant Escherichia coli by introducing the Pseudomonas sp. 61-3 PHA synthase gene (phaC2) and the maoC genes from Pseudomonas putida, Sinorhizobium meliloti, and Ralstonia eutropha. The metabolic link between fatty acid β-oxidation pathway and PHA biosynthesis pathway was constructed by MaoC, which is homologous to P. aeruginosa (R)-specific enoyl-CoA hydratase (PhaJ1). When the E. coli W3110 strains expressing the phaC2 gene and one of the maoC genes from P. putida, Sinorhizobium meliloti, and Ralstonia eutropha were cultured in LB medium containing 2 g/L of sodium decanoate as a carbon source, MCL-PHA that mainly consists of 3-hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), was produced. The monomer composition of PHA and PHA contents varied depending on MaoC employed for the production of PHA. The highest PHA content of 18.7 wt% was achieved in recombinant E. coli W3110 expressing the phaC2 gene and the P. putida maoC gene. These results suggest that MCL-PHA biosynthesis pathway can be constructed in recombinant E. coli strains from the b-oxidation pathway by employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Lee, S. Y. / 1996 / Bacterial polyhydroxyalkanoates / Biotechnol. Bioeng 49 : 1 ~ 14
2. [학술지(정기간행물)] - Madison, L. L. / 1999 / Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic / Microbiol. Mol. Biol. Rev 63 : 21 ~ 53
3. [학술지(정기간행물)] - Steinbüchel, A. / 1995 / Diversity of bacterial polyhydroxyalkanoic acid / FEMS Microbiol. Lett 128 : 219 ~ 228
4. [학술지(정기간행물)] - Qi, Q. / 1997 / Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2 / FEMS Microbiol. Lett 157 : 155 ~ 162
5. [학술지(정기간행물)] - Langenbach, S. / 1997 / Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate)synthesis / FEMS Microbiol. Lett 150 : 303 ~ 309
2. [학술지(정기간행물)] - Madison, L. L. / 1999 / Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic / Microbiol. Mol. Biol. Rev 63 : 21 ~ 53
3. [학술지(정기간행물)] - Steinbüchel, A. / 1995 / Diversity of bacterial polyhydroxyalkanoic acid / FEMS Microbiol. Lett 128 : 219 ~ 228
4. [학술지(정기간행물)] - Qi, Q. / 1997 / Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2 / FEMS Microbiol. Lett 157 : 155 ~ 162
5. [학술지(정기간행물)] - Langenbach, S. / 1997 / Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate)synthesis / FEMS Microbiol. Lett 150 : 303 ~ 309
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