Purpose: The purpose of this study was to explore the effect of glove changing during surgery for colon cancer on reducing the degree of contamination of surgical gloves. Methods: The randomized posttest control group design was used. Total 72 surgeries of colon cancer in the K University Hospital in Seoul performed by the team of A-Surgeon and B-Scrub nurse were randomly assigned to one of the three groups. Glove changing with single- gloved state was implemented in the Experimental Group I and outer glove changing with double-gloved state was executed in the Experimental Group Ⅱ. Single-gloved state was carried in the Control Group. Following the surgical procedure, specimens for bacterial culture were collected the from scrub nurse’s gloves to compare the degree of contamination among the three groups. Data were analyzed with One-way ANOVA and the Scheffe?’s multiple comparison test. Results: The degrees of contamination of the Experimental Group I and Ⅱ were significantly lower than that of the Control Group. There was no significant difference between Experimental Group I and Ⅱ. Conclusion: Glove changing during surgery with either single or double-gloved state is effective in reducing the degree of contamination of surgical gloves, therefore these methods should be utilized in clinical practice.