Various surface modification techniques are being used to improve the interfacial properties between human cells and the Ti-based implant surface. Titanium mesh is used in supporting the membrane, providing the necessary space for bone regeneration. This study was performed to investigate the bioactivity of anodized and precalcified titanium mesh. Mesh plate of Ti was anodized at 20 V for 60 minutes in a glycerol solution containing 1wt% NH₄F and 20 wt% deionized water. Heat treatment was achieved in a furnace heated to 500℃ for 2 hours. Precalcification treatment was carried out by soaking in NaH₂PO₄ solution at 80℃ for 30 minutes and saturated Ca(OH)₂ solution at 100℃ for 30 minutes. Results of MMT assay, nanotubular TiO₂ layer showed the highly ordered dense structure and cell viavility by interposing small diameter nanotubes between large ones, the shape of nanotubes was enlarged as going down. Therefore, the cell viability of anodized titanium mesh plate was more improved by precalcification treatment in 0.5 M Na₂HPO₄ and saturated Ca(OH)₂ solution. therefor, The bioactivity of titanium mesh plate was enhanced by anodic oxidation and precalcification treatment.