지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Journal of the Korean Surgical Society Vol.85 No.5
- 발행연도
- 2013.11
- 수록면
- 230 - 235 (6page)
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초록· 키워드
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Purpose: This study aimed to assess the cytolytic activity and the phenotype of circulating blood immune cells in cancer patients by using a simple preparation of peripheral blood mononuclear cells (PBMCs).
Methods: Peripheral blood was obtained from 94 diagnosed colorectal cancer (CRC) patients and 112 healthy donors. PBMCs were cocultured with K562 cells for 2 hours and lactate dehydrogenase released from the dead K562 cells was measured by using a spectrophotometer. Meanwhile, PBMCs were stained with fluorescence conjugated monoclonal antibodies (mAbs) and analyzed by flow cytometry.
Results: The cytolytic activity of PBMCs were significantly different between CRC patient and healthy groups (8.82% ± 3.84% vs. 17.51% ± 8.57%; P < 0.001). However, no significant difference in the cytolytic activity was observed after surgery in the CRC patient group (before surgery, 8.82% ± 3.84% vs. after surgery, 9.95% ± 4.94%; P = 0.326). In addition, the percentage of peripheral blood natural killer cells was significantly higher in the preoperative patient group than in the healthy group (19.97% ± 11.51% vs. 15.60% ± 5.77%, P = 0.041). In contrast, the percentage of peripheral blood lymphocytes was lower in the preoperative patient group than in the healthy group (28.41% ± 8.31% vs. 36.4% ± 8.6%, P < 0.001).
Conclusion: These results demonstrate that circulating blood immune cells of CRC patients are functionally impaired and undergo an immunophenotypic perturbation, and show that a simple preparation of PBMCs can be useful to evaluate cellular immunity in cancer.
Methods: Peripheral blood was obtained from 94 diagnosed colorectal cancer (CRC) patients and 112 healthy donors. PBMCs were cocultured with K562 cells for 2 hours and lactate dehydrogenase released from the dead K562 cells was measured by using a spectrophotometer. Meanwhile, PBMCs were stained with fluorescence conjugated monoclonal antibodies (mAbs) and analyzed by flow cytometry.
Results: The cytolytic activity of PBMCs were significantly different between CRC patient and healthy groups (8.82% ± 3.84% vs. 17.51% ± 8.57%; P < 0.001). However, no significant difference in the cytolytic activity was observed after surgery in the CRC patient group (before surgery, 8.82% ± 3.84% vs. after surgery, 9.95% ± 4.94%; P = 0.326). In addition, the percentage of peripheral blood natural killer cells was significantly higher in the preoperative patient group than in the healthy group (19.97% ± 11.51% vs. 15.60% ± 5.77%, P = 0.041). In contrast, the percentage of peripheral blood lymphocytes was lower in the preoperative patient group than in the healthy group (28.41% ± 8.31% vs. 36.4% ± 8.6%, P < 0.001).
Conclusion: These results demonstrate that circulating blood immune cells of CRC patients are functionally impaired and undergo an immunophenotypic perturbation, and show that a simple preparation of PBMCs can be useful to evaluate cellular immunity in cancer.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (24)
참고문헌 신청
1. [학술지(정기간행물)] - Jemal A / 2011 / Global cancer statistics / CA Cancer J Clin 61 : 69 ~ 90
2. [학술지(정기간행물)] - Wu AH / 1987 / Alcohol, physical activity and other risk factors for colorectal cancer: a prospective study / Br J Cancer 55 : 687 ~ 694
3. [학술지(정기간행물)] - Chan AT / 2010 / Primary prevention of colorectal cancer / Gastroenterology 138 : 2029 ~ 43.e10
4. [학술지(정기간행물)] - Cao Y / 2011 / Cancer research: past, present and future / Nat Rev Cancer 11 : 749 ~ 754
5. [학술지(정기간행물)] - Rosenberg SA / 2001 / Progress in human tumour immunology and immunotherapy / Nature 411 : 380 ~ 384
2. [학술지(정기간행물)] - Wu AH / 1987 / Alcohol, physical activity and other risk factors for colorectal cancer: a prospective study / Br J Cancer 55 : 687 ~ 694
3. [학술지(정기간행물)] - Chan AT / 2010 / Primary prevention of colorectal cancer / Gastroenterology 138 : 2029 ~ 43.e10
4. [학술지(정기간행물)] - Cao Y / 2011 / Cancer research: past, present and future / Nat Rev Cancer 11 : 749 ~ 754
5. [학술지(정기간행물)] - Rosenberg SA / 2001 / Progress in human tumour immunology and immunotherapy / Nature 411 : 380 ~ 384
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