지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 243 - 247 (5page)
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초록· 키워드
오류제보하기
Using Bacillus subtilis spore display system, with cotG as an anchoring motif, levansucrase from Zymomonas mobilis, was displayed on the outer surface of Bacillus subtilis spore. Flow cytometry of DB104 (pSDJH-cotG-levU) spore, proved the surface localization of CotG-LevU fusion protein on the spore compared to that of DB104. Enzymatic activity of DB104 (pSDJH-cotG-levU) spore showed more than 1.5 times higher levansucrase specific activity compared to that of the host spore, which is a remarkable increase of enzymatic activity considering the existence of sacA (sucrase) and sacB (levansucrase) in the Bacillus subtilis chromosome. The spore integrity, revealed by sporulation frequency test after heat and lysozyme treatment of spore, did not changed at all in spite of the CotG-LevU fusion protein incorporation into the spore coat layer during spore formation process. These data prove again that Bacillus subtilis spore could be considered as good live immobilization vehicle for efficient bioconversion process.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
References
참고문헌 (15)
참고문헌 신청
Boder, E. T / 1997 / Yeast surface display for screening combinatorial polypeptide libraries / Nat. Biotechnol 15:553~557
Jung, H. C / 1998 / Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae / Nat. Biotechnol 16:576~580
Georgiou, G. / 1997 / Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines / Nat. Biotechnol 15:29~34
Richins, R. D / 1997 / Biodegradation of organophosphorus pesticides by surfaceexpressed organophosphorus hydrolase / Nat. Biotechnol 15:984~987
Sousa, C / 1996 / Enhanced metalloadsorption of bacterial cells displaying poly-His peptides / Nat. Biotechnol 14:1017~1020
Jung, H. C / 1998 / Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae / Nat. Biotechnol 16:576~580
Georgiou, G. / 1997 / Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines / Nat. Biotechnol 15:29~34
Richins, R. D / 1997 / Biodegradation of organophosphorus pesticides by surfaceexpressed organophosphorus hydrolase / Nat. Biotechnol 15:984~987
Sousa, C / 1996 / Enhanced metalloadsorption of bacterial cells displaying poly-His peptides / Nat. Biotechnol 14:1017~1020
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UCI(KEPA) : I410-ECN-0101-2014-400-002390628