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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 465 - 470 (6page)
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Twenty six microorganisms were isolated from soil and horse manure samples from in Iowa, U.S. Microorganisms were cultivated and screened by using plate count agar (PCA) at 35℃ containing 1% (w/v) oat spelt xylan instead of glucose. The xylanase activities of bacterial strains were analyzed by measuring the concentration of reducing sugar by DNS method. All isolated strains were characterized as the rod form and gram positive strains. Among the isolated strains, the HM6 strains gave the highest xylanase activity. This strain was identified as Bacillus pumilus HM6 by 16S rDNA sequence, morphological and biochemical analysis. Optimal culture temperature and initial medium pH for B. pumilus HM6 were 30-35℃ and pH 6-7, respectively. The maximum xylanase activity of 6879 IU/mL was obtained after growth of HM6 with 1% (w/v) oat spelt xylan at 35℃ for 6 days. Studies on enzymatic properties showed that the optimum conditions for the highest xylanase activity were 60℃ and pH 8.0. In addition, xylanase activity was stable over 2 hours at 50℃, whereas activity decreased after 30 min at 70℃.
목차
Abstract
1. 서론
2. 재료 및 방법
3. 결과 및 고찰
4. 결론
References
참고문헌 (26)
참고문헌 신청
1. [학술지(정기간행물)] - Shallom, D / 2003 / Microbial hemicellulases / Curr. Opin. Microbiol 6 : 219 ~ 228
2. [학술지(정기간행물)] - 이란희 / 2009 / 꿀벌(Apis mellifera)의 장내 세균인 Bacillus sp. HY-20이 분비하는 Xylanase의 특성 / 미생물학회지 45 (4) : 332 ~ 338
3. [학술지(정기간행물)] - Chatterjee, A / 2010 / Production of xylanase in transgenic tobacco for industrial use in bioenergy and biofuel applications / Vitro Cell. Dev. Biol.-Plant 46 : 198 ~ 209
4. [학술지(정기간행물)] - LEE, JUN-HO / 2006 / Xylanase Production by Bacillus sp. A-6 Isolated from Rice Bran / Journal of Microbiology and Biotechnology 16 (12) : 1856 ~ 1861
5. [학술지(정기간행물)] - 김민정 / 2008 / Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성 / 한국동물자원과학회지 50 (3) : 429 ~ 436
2. [학술지(정기간행물)] - 이란희 / 2009 / 꿀벌(Apis mellifera)의 장내 세균인 Bacillus sp. HY-20이 분비하는 Xylanase의 특성 / 미생물학회지 45 (4) : 332 ~ 338
3. [학술지(정기간행물)] - Chatterjee, A / 2010 / Production of xylanase in transgenic tobacco for industrial use in bioenergy and biofuel applications / Vitro Cell. Dev. Biol.-Plant 46 : 198 ~ 209
4. [학술지(정기간행물)] - LEE, JUN-HO / 2006 / Xylanase Production by Bacillus sp. A-6 Isolated from Rice Bran / Journal of Microbiology and Biotechnology 16 (12) : 1856 ~ 1861
5. [학술지(정기간행물)] - 김민정 / 2008 / Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성 / 한국동물자원과학회지 50 (3) : 429 ~ 436
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UCI(KEPA) : I410-ECN-0101-2014-400-002405640