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논문 기본 정보

자료유형
학술저널
저자정보
Yun Soo Jang (Yonsei University College of Medicine) Kyoung-Ju Song (Yonsei University College of Medicine) Ju Young Kim (Yonsei University College of Medicine) Young Ah Lee (Yonsei University College of Medicine) Kyeong Ah Kim (Yonsei University College of Medicine) Sang Kyou Lee (Yonsei University) Myeong Heon Shin (Yonsei University College of Medicine)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.49 No.2
발행연도
2011.6
수록면
177 - 180 (4page)

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Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and μ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and μ-calpain may be involved in colon epithelial cell death induced by E. histolytica.

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UCI(KEPA) : I410-ECN-0101-2014-510-002511646